High level expression and facile purification of proteins, pepti

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Fusion protein or fusion polypeptide

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

4241841, 4241851, 4241861, 4241931, A61K 3900, A61K 39385

Patent

active

059895543

ABSTRACT:
A process for producing and purifying peptides and for producing peptide/protein antigens for antibody production is described. The process utilizes fusion proteins and specifically fusion proteins in which the fusion protein carrier segment includes an amino acid sequence at least about 65 amino acids long and in which the amino acid sequence does not contain negative or positive charged side chains of amino acids.

REFERENCES:
patent: 4512922 (1985-04-01), Jones et al.
patent: 4743679 (1988-05-01), Cohen et al.
patent: 4952496 (1990-08-01), Studier et al.
patent: 5008373 (1991-04-01), Kingsman et al.
patent: 5043158 (1991-08-01), Sleytr et al.
patent: 5302526 (1994-04-01), Keck et al.
patent: 5322769 (1994-06-01), Bolling et al.
patent: 5322930 (1994-06-01), Tarnowski et al.
Duong, Le T.; Caulfield, Michael P., and Rosenblatt, Michael, Synthetic Signal Peptide and Analogs Display Different Activities in Mammalian and Plant in Vitro Secretion Systems, The Journal of Biological Chemistry (May 5, 1982), Baltimore, MD, USA, v262, No. 13, pp. 6328-6333.
Lin, Shu-Wha; Dunn, John J.; Studier, F. William; and Stafford, Darrel W., Expression of Human Factor IX and Its Subfragments in Escherichia coli and Generation of Antibodies to the Subfragments, Biochemistry (1987), v26, pp. 5267-5274.
Ngsee, Johnny K.; Hansen, William; Walter, Peter; and Smith, Michael, Cassette Mutagenic Analysis of the Yeast Invertase Signal Peptide: Effects on Protein Translocation, Molecular and Cellular Biology (Aug. 9, 1989), Washington, DC, US, v9, No. 8, pp. 3400-3410.
Randall, L.L. and Hardy, S.J.S., Unity in Function in the Absence of Consensus in Sequence: Role of Leader Peptides in Export, Science (Mar. 3, 1989), Washington, D.C., USA, v243, pp. 1156-1159.
Tamamoto, Yoshio; Taniyama, Yoshio; Kikuchi, Masakazu; and Ikehara, Morio, Engineering of the Hydrophobic Segment of the SignalSequence For Efficient Secretion of Human Lysozyme by Saccharomyces cerevisiae; Biochemical and Biophysical Research Communications (Dec. 16, 1987), Duluth, MN, USA, v149, No. 2, pp. 431-436.
Bolivar et al, (1977), Gene 2:95.
Clewell, D. R. et al, (1969), Proc Nat Acad Sci USA 62: 1159.
Cohen, S.N., (1972), Proc Nat Acad Sci USA 69: 2110.
Current Protocols in Molecular Biology (1994); chapter 16.
Klein, J., (1990) Immunology, Blackwell Scientific Publications, Inc. Massachusetts, 269-293.
Kohler & Milstein, (1975), Nature, 256: 495.
Lin, S. et al., (1987), Biochemistry, 26: 5267-5274.
Sano, C. et al., (1992), Science 258: 120-122.
Smith, A. J. et al., (1992), Techniques in Protein Chemistry III, 219-229.
Studier F. W., et al, (1990), Meth. Enzym. 185: 60-89.
Tae H. J., et al., (1983), Methods in Enzymology (Chapter 51), Academic Press, Inc., 91: 580-609.
Hey et al. (1994) A two fusion partner system for raising antibodies against small immunogens expressed in bacteria. Journal of Immunological Methods 173(2): 149-156, Aug. 1994.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

High level expression and facile purification of proteins, pepti does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with High level expression and facile purification of proteins, pepti, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and High level expression and facile purification of proteins, pepti will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1218263

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.