High gel strength low electroendosmosis agarose

Details High gel strength low electroendosmosis agarose High gel strength low electroendosmosis agarose

1989-08-03

1991-01-08

Niebling, John F.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, 2523153, 106205, 536 3, 536114, 536127, C07K 114, C07K 314, B01D 5702

Patent

active

049832689

ABSTRACT:
Purified agarose suitable for rapid electrophoresis, characterized by a sulfate content of less than 0.2 wt % but greater than zero, a pyruvate content of 0-0.1 wt %, and a nitrogen content of 0-0.02 wt %. Gels prepared from the agarose exhibit a gel strength at 1.0 wt % concentration of at least 1200 g/cm.sup.2, substantial absence of DNA binding in 0.07 M or less tris acetate buffer, and an electroendosmosis (EEO) at 1.0 wt % concentration of 0.05 or less. Agaroses are purified to provide the low EEO material by dissolving agarose or alkali-modified agar in an aqueous medium buffered at a pH of 6.0 to 8.0 and containing no more than 2.0 nM salt as chloride, and precipitating the agarose by contact with a lower alkanol.

REFERENCES:
patent: 4290911 (1981-09-01), Cook et al.
Bio-Rad, "Price List L", Jan. 1976, p. 172.
Zajic, "Properties and Products of Algae", 1970, pp. 57-60; p. 143.

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