Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...
Patent
1997-01-03
1998-06-16
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an organic...
435177, 435181, 435815, 436528, 436530, 436532, 530402, 530413, 530812, 530814, 530816, C12N 1112, G01N 33544, C07K 1706, C07K 1712
Patent
active
057669082
ABSTRACT:
An affinity support is provided containing a high flux semipermeable hydrogel membrane surface-modified with an affinity ligand, such as a protein which may be an antibody or enzyme, or a cell receptor complement, useful for affinity separation of biological macromolecules, including insoluble proteins, cells, and cell fragments, from solution. The exclusion limit (molecular weight cut-off) of the matrix is selected to substantially restrict immobilized protein or other ligand to the surface thereof for maximization of available ligand binding capacity. The exclusion limit is also selected to permit reagent(s) used for the matrix/ligand linkage to penetrate into and form covalent bonds with the membrane on the interior surfaces of the membrane for optimizing packing densities of the affinity ligand exterior to the membrane. By this substantially complete orientation of affinity ligand between affinity matrix and solution interface, the recognition sites of the proteins or other ligands are maximally available to complementary molecules or cells bearing complementary determinants for complexing therewith.
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Klein Elias
Yeager Donald H.
Akzo Nobel nv
Naff David M.
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