High fidelity thermostable ligase and uses thereof

Details High fidelity thermostable ligase and uses thereof High fidelity thermostable ligase and uses thereof

2005-09-27

2005-09-27

Hutson, Richard (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

C435S194000, C435S193000, C530S350000

Reexamination Certificate

active

06949370

ABSTRACT:
The present invention is directed to a thermostable ligase having substantially higher fidelity than either T4 ligase orThermus thermophilusligase. The DNA molecule encoding this enzyme as well as expression systems and host cells containing it are also disclosed. The thermostable ligase of the present invention is useful in carrying out a ligase detection reaction process and a ligase chain reaction process.

REFERENCES:
patent: 4683202 (1987-07-01), Mullis
patent: 4883750 (1989-11-01), Whiteley et al.
patent: 5470705 (1995-11-01), Grossman et al.
patent: 5494810 (1996-02-01), Barany et al.
patent: 5496699 (1996-03-01), Sorenson
patent: 5506137 (1996-04-01), Mathur et al.
patent: 5516663 (1996-05-01), Backman et al.
patent: 5728526 (1998-03-01), George, Jr. et al.
patent: 5912148 (1999-06-01), Eggerding
patent: 6054564 (2000-04-01), Barany et al.
patent: 6312892 (2001-11-01), Barany et al.
patent: 6576453 (2003-06-01), Barany et al.
patent: WO 91/17239 (1991-11-01), None
patent: WO 93/17126 (1993-09-01), None
patent: WO 97/31256 (1997-08-01), None
patent: WO 97/45559 (1997-12-01), None
patent: WO 98/03673 (1998-01-01), None
patent: WO 00/26381 (2000-05-01), None
Ngo et al., Computational Complexity, Protein Structure Prediction, and the Levinthal Paradox, in The Protein Folding Problem and Tertiary Structure Prediction, 1994, Merz et al. (ed.), Birkhauser, Boston, MA, pp. 443 and 492-495.
Tong et al., Biochemical properties of a high fidelity DNA ligase from Thermus species AK16D, Nucleic Acids Research, vol. 27, No. 3, pp 788-794, 1999.
Luo, J. et al., “Improving the fidelity of Thermus thermophilus DNA ligase”, Nucl. Acids Res., vol. 24, pp. 3071-3078 (1996).
Kreiner, T., “Rapid Genetic Sequence Analysis Using a DNA Probe Array System,”American Labortory39-43 (Mar. 1996).
Kovach et al., “Mutation Detection by Highly Sensitive Methods Indicates That p53 Gene Mutations in Breast Cancer Can Have Important Prognostic Value,”Proc. Natl Acad. Sci. USA93:1093-96 (1996).
Lin et al., “Multiplex Genotype Determination at a Large Number of Gene Loci,”Proc. Natl. Acad. Sci. USA93:2582-87 (1996).
Tavormina et al., “Thanatophoric Dysplasia (Types I and II) Caused by Distinct Mutations in Fibroblast Growth Factor Receptor 3,”Nature Genetics9:321-328 (1995).
Barany et al., “Cloning, Overexpression and Nucleotide Sequence of a Thermostable DNA Ligase-Encoding Gene,”Gene109:1-11 (1991).
Sidransky et al., “Identification of ras Oncogene Mutations in the Stool of Patients With Curable Colorector Tumors,”Science256:102-105 (1992).
Brennan et al., “Molecular Assessment of Histopathological Staging in Squamous-Cell Carcinoma of the Head and Neck,”The New England Journal of Medicine332(7):429-35 (1995).
Berthélemy et al., “Identification of K-ras Mutations in Pancreatic Juice in the Early Diagnosis of Pancreatic Cancer,”Annals of Internal Medicine123(3):188-91 (1995).
Hayashi et al., “Genetic Diagnosis Identifies Occult Lymph Node Metastases Undetectable by the Histopathological Method,”Cancer Research54:3853-56 (1994).
Tada et al., “Detection of ras Gene Mutations in Pancreatic Juice and Peripheral Blood of Patients with Pancreatic Adenocarcinoma,”Cancer Research53:2472-74 (1993).
Tada et al., “Clinical Application of ras Gene Mutation for Diagnosis of Pancreatic Adenocarcinoma,”Gastroenterology100:233-38 (1991).
Mitsudomi et al., “Mutations of ras Genes Distinguish a Subset of Non-Small-Cell Lung Cancer Cell Lines From Small-Cell Lung Cancer Cell Lines,”Oncogene6:1353-62 (1991).
Day et al., “Detection of Steroid 21-Hydroxylase Alleles Using Gene-Specific PCR and a Multiplexed Ligation Detection Reaction,”Genomics29:152-62 (1995).
Frenkel et al., “Specific, Sensitive, and Rapid Assay for Human Immunodeficiency Virus Type 1 pol Mutations Associated With Resistance to Zidovudine and Didanosine,”J. Clin. Microbiol. 33(2):342-47 (1995).
Abravaya et al., “Detection of Point Mutations With a Modified Ligase Chain Reaction (Gap-LCR),”Nucleic Acids Research23(4):675-82 (1995).
Powell et al., “Molecular Diagnosis of Familial Adenomatous Polyposis,”The New England Journal of Medicine320(27):1982-87 (1993).
Jen et al., “Molecular Determinants of Dysplasia in Colorectal Lesions,”Cancer Research54:5523-26 (1994).
Redston et al., “Common Occurrence of APC and K-ras Gene Mutations in the Spectrum of Colitis-Associated Neoplasias,”Gastroenterology108:383-92 (1995).
Lu et al., “Quantitative Aspects of the Mutant Analysis by PCR and Restriction Enzyme Cleavage (MAPREC),”PCR Methods and Applications3:176-80 (1993).
Kuppuswamy et al., “Single Nucleotide Primer Extension to Detect Genetic Diseases: Experimental Application to Hemophilia B (factor IX) and Cystic Fibrosis Genes,”Proc. Natl. Acad. Sci. USA88:1143-47 (1991).
Rust et al., “Mutagenically Separated PCR (MS-PCR): A Highly Specific One Step Procedure for Easy Mutation Detection,”Nucleic Acid Research21(16):3623-29 (1993).
Suzuki et al., “Dectection of ras Gene Mutations in Human Lung Cancers by Single-Strand Conformation Polymorphism Analysis of Polymerase Chain Reaction Products,”Oncogene5:1037-43 (1990).
Balles et al., “Facilitated Isolation of Rare Recombinants by Ligase Chain Reaction: Selection for Intragenic Crossover Events in theDrosophila optomotor-blindGene,”Mol. Gen. Genet. 245:734-40 (1994).
Reynolds et al., “Analysis of Genetic Markers in Forensic DNA Samples Using the Polymerase Chain Reaction,”Anal. Chem. 63:2-15 (1991)).
Buyse et al., “Rapid DNA Typing of Class II HLA Antigens Using the Polymerase Chain Reaction and Reverse Dot Blot Hybridization,”Tissue Antigens41:1-14 (1993).
Gyllensten et al., “PCR-Based HLA Class II Typing,”PCR Meth. Appl. 1:91-98 (1991).
Chamberlain et al., “Deletion Screening of the Duchenne Muscular Dystrophy Locus Via Multiplex DNA Amplification,”Nucleic Acids Res. 16:11141-56 (1988).
Tsui, L. C., “Mutations and Sequence Variations Detected in the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Gene: A Report From the Cystic Fibrosis Genetic Analysis Consortium,”Human Mutat. 1:197-203 (1992).
Hollstein et al., “p53 Mutations in Human Cancers,”Science253:49-53 (1991).
Saiki et al., “Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia,”Science230:1350-1354 (1985).
Wu et al., “The Ligation Amplification Reaction (LAR)—Amplification of Specific DNA Sequences Using Sequential Rounds of Template-Dependent Ligation,”Genomics4:560-69 (1989).
Landegren et al., “A Ligase-Mediated Gene Detection Technique,”Science241:1077-80 (1988).
Winn-Deen, et al., “Sensitive Fluorescence Method for Detecting DNA Ligation Amplification Products,”Clinical Chemistry37(9):1522-23 (1991).
Barany, F., “Genetic Disease Detection and DNA Amplification Using Cloned Thermostable Ligase,”Proc. Nat'l Acad. Sci. USA88:189-93 (1991).
Grossman et al., “High-Density Multiplex Detection of Nucleic Acid Sequences: Oligonucleotide Ligation Assay and Sequence-Coded Separation,”Nucl. Acids Res. 22(21):4527-37 (1994).
Jou et al., “Deletion Detection in the Dystrophin Gene by Multiplex Gap Ligase Chain Reaction and Immunochromatographic Strip Technology,”Human Mutation5:86-93 (1995).
Gibbs et al., “Detection of Single DNA Base Differences by Competitive Oligonucleotide Priming,”Nucleic Acids Res. 17:2437-48 (1989).
Chehab et al., “Detection of Specific DNA Sequences by Fluorescence Amplification: A Color Complementation Assay,”Proc. Natl. Acad. Sci. USA86:9178-82 (1989).
Livak et al., “Detection of Single Base Differences Using

Affiliated with

Also associated with

Rating

High fidelity thermostable ligase and uses thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with High fidelity thermostable ligase and uses thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and High fidelity thermostable ligase and uses thereof will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3410490