High fidelity reverse transcriptases and uses thereof

Details High fidelity reverse transcriptases and uses thereof High fidelity reverse transcriptases and uses thereof

2006-06-06

2006-06-06

Fredman, Jeffrey (Department: 1637)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

C435S183000, C435S091100, C435S006120

Reexamination Certificate

active

07056716

ABSTRACT:
The invention relates to reverse transcriptases which have increased fidelity (or reduced misincorporation rate) and/or terminal deoxynucleotidyl transferase activity. In particular, the invention relates to a method of making such reverse transcriptases by modifying or mutating specified positions in the reverse transcriptases. The invention also relates to nucleic acid molecules containing the genes encoding the reverse trancriptases of the invention, to host cells containing such nucleic acid molecules and to methods to make the reverse trancriptases using the host cells. The reverse transcriptases of the invention are particularly suited for nucleic acid synthesis, sequencing, amplification and cDNA synthesis.

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