High eicosapentaenoic acid producing strains of Yarrowia...

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi

Reexamination Certificate

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C435S254110, C435S134000, C435S189000, C435S193000, C536S023200

Reexamination Certificate

active

07932077

ABSTRACT:
Engineered strains of the oleaginous yeastYarrowia lipolyticacapable of producing greater than 25% eicosapentaenoic acid (EPA, an ω-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of EPA. Production host cells are claimed, as are methods for producing EPA within said host cells.

REFERENCES:
patent: 5244921 (1993-09-01), Kyle et al.
patent: 5246841 (1993-09-01), Yazawa et al.
patent: 5246842 (1993-09-01), O'Brien et al.
patent: 5401646 (1995-03-01), Shinmen et al.
patent: 6136574 (2000-10-01), Knutzon et al.
patent: 6331568 (2001-12-01), Horrobin
patent: 6624195 (2003-09-01), Horrobin
patent: 7259255 (2007-08-01), Picataggio et al.
patent: 2005/0216975 (2005-09-01), Yadav et al.
patent: 2006/0195939 (2006-08-01), Damude et al.
patent: WO 00/34439 (2000-06-01), None
patent: WO 2004/101757 (2004-11-01), None
patent: WO 2004/104167 (2004-12-01), None
patent: WO 2005/047485 (2005-05-01), None
Characterization and comparison of fatty acyl Delta6 desaturase cDNAs from freshwater and marine teleost fish species. Comp Biochem Physiol B Biochem Mol Biol. Oct. 2004;139(2):269-79.
Janice McColl, Health Benefits of Omega-3 Fatty Acids, Nutraceuticals. vol. 2(4):35-40, 2003.
Frederic Domergue et al., Cloning and Functional Characterization of Phaeodactylum Tricornutum Front-End Desaturases Involved in Eicosapentaenoic Acid Biosynthesis, Eur. J. Biochem., vol. 269:4105-4113, 2002.
Baoxiu al et al., Production of Very Long Chain Polyunsaturated Omega-3 and Omega-6 Fatty Acids in Plants, Nature Biotechnology, vol. 22(6):739-745.
J. M. Dyer et al., Metabolic Engineering ofSaccharomyces cerevisiaefor Production of Novel Lipid Compounds, Appl. Microbiol. Biotechnol., vol. 59:224-230, 2002.
Baoxiu Qi et al., Identification of a CDNA Encoding a Novel C18-DELTA9 Polyunsaturated Fatty Acid-Specific Elongating Activity From the Docosahexaenoic Acid (DHA)-Producing Microalga,Isochrysis galbana, FEBS Letters, vol. 510:159-165, 2002.
James G. Wallis et al., The DELTA8-Desaturase ofEuglena gracilis: An Alternate Pathway for Synthesis of 20-Carbon Polyunsaturated Fatty Acids, Archives of Biochemistry and Biophysics, vol. 365(2):307-316, 1999.
Casimir C. Akoh et al., Healthful Lipids, AOCS: Champaign, IL, 2005; Chapters 14-16.
Juretzek, Thomas et al., Vectors for gene expression and amplification in the yeastYarrowia lipolytica, Yeast, 2001, p. 97-113, vol. 18, John Wiley & Sons, Ltd.
Sayanova, Olga V. et al., Eicosapentaenoic acid: biosynthetic routes and the potential for synthesis in transgenic plants, Phytochemistry, 2004, p. 147-158, vol. 65, Elsevier Ltd.
Hobbs, Douglas H. et al., Cloning of a cDNA encoding diacylglycerol acyltransferase fromArabidopsis thalianaand its functional expression, FEBS Letters, 1999, p. 145-159, vol. 452, Federal of European Biochemical Societies.

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