High efficiency translation of mRNA molecules

Details High efficiency translation of mRNA molecules High efficiency translation of mRNA molecules

1996-02-12

1998-09-15

Degen, Nancy

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

430 711, 430 712, 430183, 536 231, 536 241, 536 242, C12P 2102, C07H 2104, C12N 1567

Patent

active

058077073

ABSTRACT:
An increased level of translation of a selected mRNA molecule is effected by coupling specific nucleotide sequences at the 5'- and 3' -ends of a nucleic acid molecule transcribable to or which itself is the mRNA molecule. The nucleotide sequence at the 5'-end is effective to increase the rate of translation initiation of the mRNA molecule in a cell while the nucleotide sequence at the 3'-end is effective to increase the period of translation of the mRNA molecule in a cell. The nucleotide sequence of the 3'-end is provided by a 3'-untranslated region (3'-UTR) of a gene, particularly that of .beta.-prolactin, or an effective fragment thereof. A polyadenylation sequence preferably is provided at the 3'-end of the 3'-UTR sequence. The 3'-UTR sequence provides mRNA stabilization independent of the poly A tail.

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