Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2011-04-26
2011-04-26
Duffy, Patricia A (Department: 1645)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C800S291000
Reexamination Certificate
active
07932088
ABSTRACT:
The present invention provides molecular methods for efficiently transforming the genome of common disease-transmitting parasites, such asPlasmodium falciparum. The transformation efficiencies are improved up to 100 times over those conventionally known. The methods provide high saturation of the target parasite genome, of 50% or greater, and target non-specifically TTAA-rich sites in the parasite genome. The invention also discloses a model that may be used to functionally annotate the genome of thePlasmodium falciparum, thus permitting the design and screening of compounds that may be useful in the control and inhibiting of diseases caused and transmitted by these parasites, including malaria. Highly efficient and multi-site integrating transposons, particularly piggyBac transposons, which provide for random and multi-site integration into parasite genomes in the presence of a helper plasmid, are also presented.
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Adams John H.
Balu Bharath
Fraser Malcolm J.
Shoue Douglas A.
Archie Nina A
Duffy Patricia A
Katten Muchin & Rosenman LLP
Mayfield Denise L.
University of Notre Dame du Lac
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