Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se
Patent
1996-03-13
2000-03-21
Saunders, David
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Primate cell, per se
435 2, 435373, 435374, 435375, 435377, 435383, 435384, 4353201, 435455, 530351, 53038875, 536 231, 536 2372, C12N 510, C12N 1511, C07K 1455, C07K 1628
Patent
active
06040177&
ABSTRACT:
The present invention provides a rapid expansion method (termed "REM"), for quickly generating large numbers of T lymphocytes, including cytolytic and helper T lymphocytes. REM involves culturing the T cells in association with a disproportionately large concentration of nondividing feeder cells, preferably .gamma.-irradiated peripheral blood mononuclear cells ("PBMC") present at an excess of at least 40-fold (relative to the number of target T cells), more preferably at an excess of at least about 200-fold. Cultures grown under REM exhibit dramatically enhanced expansion rates that can be even further elevated by the use of appropriate concentrations of an additional feeder cell, an anti-CD3 monoclonal antibody and IL-2, as described herein. Clonal expansions in the range of 500-fold to 3000-fold can be achieved within a single stimulation cycle of about 10-13 days, which is more than 100-fold more efficient than currently employed methods of culturing human T cell clones. Genetic transduction efficiencies were also enhanced using REM-expanded T lymphocytes. Several examples involving human bone marrow transplant recipients illustrate the effective use of REM-expanded antigen-specific cytotoxic T lymphocytes for adoptive immunotherapy in humans.
REFERENCES:
patent: 4544632 (1985-10-01), Yamamura et al.
patent: 4675291 (1987-06-01), Yamamura et al.
patent: 4839290 (1989-06-01), Kaieda et al.
patent: 5057423 (1991-10-01), Hiserodt et al.
patent: 5399346 (1995-03-01), Anderson et al.
Morecki, S et al. Cancer Immunol Immunother. 32:342-352, 1991.
Yang, Z et al. Mol Cell Biol. 7(11):3923-3928 Nov. 1987.
Tiberghein, P et al. Blood. 84(4):1333-1341, Aug. 15, 1994.
Greenberg, "Adoptive T cell therapy of tumors: Mechanisms operative in the recognition and elimination of tumor cells" Adv. Immunol. Dixon, F.J. ed., Academic Press, Inc. Press, Inc. New York, (1991) 49:281-355.
Riddell et al., "Restoration of viral immunity in immunodeficient humans by the adoptive trnasfer of T cell clones" Science (1992) 257:238-241.
Reusser et al., "Cytotoxic T-lymphocyte response to cytomegalovirus after human allogeneic bone marrow transplantation: Pattern of recovery and correlation with cytomegalovirus infection and disease" Blood (1991) 78:1373-1380.
Riddell et al., "Class I MHC-restricted cytotoxic T lymphocyte recognition of cells infected with human cytomegalovirus does not require endogenous viral gene expression" J. Immunol. (1991) 146:2795-2804.
Riddell et al., "The use of anti-CD3 and anti-CD28 monoclonal antibodies to clone and expand human antigen-specific T cells" J. Immunol. Meth. (1990) 128:189-201.
Rosenberg et al., "A progress report on the treatment of 157 patients with advanced cancer using lymphokine-activated killer cells and interleukin-2 or high-dose interleukin-2 alone" New Engl. J. Med. (1987) 316:890-897.
Rosenberg et al., "Use of tumor-infiltrating lymphocytes and interleukin-2 in the immunotherapy of patients with metastatic melanoma" New Engl. J. Med. (1988) 319:1676-1680.
Ho et al., "A phase I study of adoptive transfer of autologous CD8.sup.+ T lymphocytes in patients with acquired immunodeficiency syndrome (AIDS)--related complex or AIDS" Blood (1993) 81:2093-2101.
Gillis et al., "Interleukin-2 dependent culture of cytolytic T cell lines" Immunol. Rev. (1981) 54:81-109.
Paul et al., "Long-term growth and cloning of non-transformed lymphocytes" Nature (1981) 294:697-699.
Lenardo, "Interleukin-2 programs mouse .alpha..beta. T lymphocytes for apoptosis" Nature (1991) 353:858-861.
Boehme et al., "Propriocidal apoptosis of mature T lymphocytes occurs at S phase of the cell cycle" Eur. J. Immunol. (1993) 23:1552-1560.
Miller, "Retroviral vectors" Current Topics in Microbiol & Immunol. (1992) 158:1-24.
Rosenberg et al., "Gene transfer into human--immunotherapy of patiens with advanced melanoma, using tumor-infiltrating lymphocytes modified by retroviral gene transduction" New Engl. J. Med. (1990) 323:570-578.
Springett et al., "Infection efficiency of T lymphocytes with amphotropic retroviral vectors is cell cycle dependent" J. Virol. (1989) 63:3865-3869.
Roe et al., "Integration of murine leukemia virus DNA depends on mitosis" EMBO J. (1993) 12:2099-2108.
Burgess et al., "The nature and action of granulocyte-macrophage colony stimulating factors" Blood(1980) 56:947-958.
Crossland et al., "T. cells from tumor-immune mice nonspecifically expanded in vitro with anti-CD3 plus IL-2 retain specific function in vitro and can eradicate disseminated leukemia in vivo" J. Immunol. (1991) 146:4414-4420.
Gilbert et al., "Selective interference with class I major histocompatibility complex presentation of the major immediate-early protein following infection with human cytomegalovirus" J. Virol. (1993) 67:3461-3469.
Moore et al., "Culture of normal human leukocytes" J. Am. Med. Assoc. (1967) 199:87-92.
Riddell et al., "Phase I study of cellular adoptive immunotherapy using genetically modified CD8.sup.+ HIV-specific T cells for HIV seropositive patients undergoing allogeneic bone marrow transplant" Human Gene Therapy (1992) 3:319-338.
Shcook et al., "Lymphokine and monokine activities in supernatants from human lymphoid and myeloid cell lines" Lymphokines (1981) 2:1-19.
Weaver et al., "Syngeneic transplantation with peripheral blood mononuclear cells collected after the administration of recombinant human granulocyte colony-stimulating factor" Blood (1993) 82:1981-1984.
Van de Griend et al., "Rapid expansion of allospecific cytotoxic T cell clones using non-specific feeder cell lines without further addition of exogenous Il2" Transplatation (1984) 38:401-406.
Van de Griend et al., "Rapid expansion of human cytotoxic T cell clones: Growth promotion by heat-labile serum component and by various types of feeder cells" J. Immunol. Meth. (1984) 66:285-298.
Weber et al., "Activation through CD3 molecule leads to clonal expansion of all human periphral blood T lymphocytes: functional analysis of clonally expanded cells" J. Immunol. (1985) 135:2337-2342.
Greenberg Philip D.
Riddell Stanley R.
Fred Hutchinson Cancer Research Center
Saunders David
VanderVegt R. Pierre
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