Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Patent
1996-09-16
1999-07-13
Railey, II, Johnny F.
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
435 4, 435 6, 4353201, 435325, 536 231, 536 234, 536 241, C12N 1564, C12N 510, C12N 1511, C12N 1579
Patent
active
059226013
ABSTRACT:
A gene trap construct for identification of genes whose activity is regulated upon a cellular transition event which comprises in downstream sequence (i) a cassette having a functional splice acceptor, a translation stop sequence and an internal ribosome entry site and (ii) a promoterless protein coding sequence encoding at least one polypeptide providing positive and negative selection traits. A method for identification of genes whose activity is regulated upon a cellular transition event by introducing the gene trap construct into a cell and observing expression of the positive and/or negative selection traits before and after the transition event.
REFERENCES:
Sugimoto et al. 1994. Efficient Expression of Drug-Selectable Genes in Retroviral Vectors Under Control of an Internal Ribosome Entry Site. Bio/technology, 12:694-698.
Alberts et al. 1989. Molecular Biology of The Cell, 2.sup.nd edn. Garland Publishing Inc., New York, p. 533.
Casadaban, M.J., and Cohen, S.N. (1979). Lactose genses fused to exogenous prometers in one step using a Mulac bacteriophage: In vivo probe for transcriptional control sequences. Proc. Natl. Acad. Sci. USA 76, 4530-4533.
Chang, W., Hubbard, S.C., Friedel, C., and Ruley, H.E. (1993). Enrichment of insertional mutants following retrovirus gene trap selection. Virology 193, 737-747.
Chu, G., and Sharp, P.A. (1981). A gene chimaera of SV40 and mouse .beta.-globin is transcribed and properly spliced. Nature 289, 378-382.
DeGregori, J., et al., e. (1994). A murine homolog of the yeast RNA 1 gene is required for postimplantation development. Genes & Development 8, 265-276.
Freidrich, G., and Soraino, P. (1991). Promoter traps in embryonic stem cells; a genetic screen to identify and mutate developmental genes in mice. Genes & Development 5, 1513-1523.
Gossler, A., Joyner, A.L., Rossant, J., and Skarnes, W.C. (1989). Mouse embryonic stem cells and reporter constructs to detect developmentally regulated genes. Science 244.
Hill, D.P., and Wurst, W. (1993). Screening for novel pattern formation genes using gene trap approaches. Methods in Enzymology 225, 664-681.
Joyner, A.L. (1991). Gene targeting and gene trap screens using embryonic stem cells; new approaches to mammalian development. BioEssays 13, 649-656.
Joyner, A.L., Auerbach, A., and Skarnes. W.C. (1992). The gene trap approach in embryonic stem cells: the potential for genetic screens in mice. Ciba Foundation Symposium 165, 277-288.
Macleod, D., Lovell-Badge, R., Jones, S. and Jackson, I. (1991). A promoter trap in embryonic stem (ES) cells selects for integration of DNA into CpG islands. Nucleic Acids Research 19, 17-23.
Mountford, P., Zevnik, B., Duwel, A., Nichols, J. Li, M., Dani, C., Robertson, M., Chambers, I., and Smith, A. (1994). Dicistronic targeting constructs: reporters and modifiers of mammalian gene expression. Proc.Natl.Acad.Sci.U.S.A. 91, 4303-4307.
Reddy, S., DeGregori, J.V., von Melchner, H., and Ruley, H.E. (1991). Retrovirus promoter-trap vector to induce lacZ gene fusions in mammalian cells. Journal of Virology 65, 1507-1515.
Reddy, S., Rayburn, H., von Melchner, H., and Ruley, H.E. (1992). Fluorescence-activated sorting of totipotent embryonic stem cells expressing developmentally regulated lacZ fusion genes. Proc. Natl. Acad. Sci. USA 89, 6721-6725.
Skarnes, W.C., Auerbach, B.A., and Joyner, A.L. (1992). A gene trap approach in mouse embryonic stem cells: the lacZ reporter is activated by splicing, reflects endogenous gene expression, and is mutagenic in mice. Genes & Development 6, 903-918.
Soriano, P., Friedrich G., and Lawinger, P. (1991). Promoter interactions in retrovirus vectors introduced into fibroblasts and embryonic stem cells. Journal of Virology 65, (5), 2314-2319.
Vaulont, S., Daines, S., and Evans, M. (1995). Disruption of the adenosine deaminase (ADA) gene using a dicistronic promoterless construct: production of an ADA-deficient homozygote ES cell line. Transgenic Research 4, 247-255.
Von Melchner, H., DeGregori, J.V., Rayburn, H., Reddy, S., Friedel, C., and Ruley, H.E. (1992). Selective disruption of genes expressed in totipotent embryonal stem cells. Genes & Development 6, 919-927.
Von Melchner, H., Reddy, S., and Ruley, H.E. (1990). Isolation of cellular promoters by using a retrovirus promoter trap. Proc. Natl. Acad. Sci. USA 87, 3733-3737.
Weber, R., de Villiers, J., and Schaffner, W. (1984). An SV40 "enhancer trap" incorporates exogenous enhancers or generates enhancers from its own sequences. Cell 36, 983-992.
Baetscher Manfred
Nir Waan-Jeng
BioTransplant, Inc.
Olstein Elliot M.
Railey II Johnny F.
Semionow Raina
LandOfFree
High efficiency gene trap selection of regulated genetic loci does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with High efficiency gene trap selection of regulated genetic loci, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and High efficiency gene trap selection of regulated genetic loci will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2275303