High efficiency gene targeting in plants

Details High efficiency gene targeting in plants High efficiency gene targeting in plants

2006-10-24

2006-10-24

Nguyen, Dave Trong (Department: 1633)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

C800S294000, C800S298000, C800S300000, C435S419000, C435S468000, C435S252200, C435S320100, C536S023100

Reexamination Certificate

active

07126041

ABSTRACT:
The present invention provides a method for the targeted insertion of a nucleotide of interest into a specific chromosomal site within a plant cell. The method comprises the steps of: (a) providing a plant cell, the plant cell optionally but preferably having a heterologous target site on a chromosome thereof, wherein said target site is flanked by at least one recombination site; and then (b) transforming said plant cell with a transformation vector (e.g., with anAgrobacteriumtransformation vector) carrying a nucleotide sequence of interest, wherein said nucleotide sequence of interest is flanked by at least one recombination site that corresponds to the recombination sites of said target site, so that said nucleotide of interest is inserted into said chromosome at said target site (when a target site is employed).

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