High efficiency fluorescence flow cell for capillary liquid chro

Optics: measuring and testing – Sample – specimen – or standard holder or support – Fluid containers

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356318, G01N 2105

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active

054305411

ABSTRACT:
A fluorescence flow cell having a capillary through which a sample fluid is moved, an optical source that directs an exposing beam of light through the sample fluid, a collector that collects fluorescent light from the exposed region of the sample and directs such collected light to a detector. A beam blocker prevents light that is scattered by the capillary walls from reaching the detector, thereby improving the signal-to-noise ratio of the system. The beam blocker also has a pair of legs that prevent the sample fluid from being exposed by either the exposing light or the collected light outside of the exposing region. The structure of the components enables the various components to be quickly and accurately assembled and enables easy replacement of the capillary.

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patent: 4675300 (1987-06-01), Zare et al.
Stephen E. Moring et al, Analytical Aspects of an Automated Capillary Electrophoresis System, LC.multidot.GC, vol. 8, No. 1.
Michael Albin et al, Fluorescence Detection in Capillary Electrophoresis: Evaluation of Derivatizing Reagents and Techniques, Analytical Chemistry, 1991, p. 63.
Alfredo E. Bruno et al, On-Column Capillary Flow Cell Utilizing Optical Waveguides for Chromatographic Applications, Anal. Chem., 1989, 61, pp. 876-883.

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