Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1992-08-12
1993-12-14
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
4351723, 4353201, C12N 1510, C12N 1564
Patent
active
052701859
ABSTRACT:
Asymmetrically tailed plasmid primers are provided, each of which comprises a cut, double-stranded DNA plasmid containing a functional origin of replication, at least one functional selection marker gene, a 3' oligo (dT) tail and a 3' oligo (dC) or oligo (dG) tail which is terminated by a phosphate group. Methods for making and using the primers for the highly efficient production of complex cloning libraries and a kit for carrying out the cloning method of the invention are also provided.
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Choi Kathleen L.
Epstein William H.
Gould George M.
Hoffmann-La Roche Inc.
Rocha Patricia S.
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