Optics: measuring and testing – By electrophoresis
Patent
1997-03-18
2000-01-11
Font, Frank G.
Optics: measuring and testing
By electrophoresis
204461, 204466, 204612, G01N 2100
Patent
active
060142138
ABSTRACT:
A high dynamic range apparatus for separation and detection of polynucleotide fragments has a housing adapted to receive an electrophoresis gel holder containing an electrophoresis gel loaded with fluorophore-labeled samples; one or more laser diodes for providing radiation of a frequency suitable for excitation of the fluorophore which irradiates a an array of excitation/detection sites on the electrophoresis gel; an array of detectors aligned with the excitation/detection sites for collecting fluorescent emissions; and one or more components for increasing the dynamic range of the instrument by at least an order of magnitude. These components, which can be used individually or in combination include detectors that are connected to a signal processing system that modulates the period of signal integration employed so that large signals are totaled at short time intervals and smaller signals are totaled at longer time intervals; the use of a beam splitter to produces a high intensity beam of emitted light and a low intensity beam of emitted light from each excitation/detection site; and a device for modulating the intensity of the excitation beam can be used to improve the dynamic range of the instrument.
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Cassidy James W.
Izmailov Alexandre M.
Renfrew John A.
Waterhouse Paul
Zaleski Henryk
Font Frank G.
Punnoose Roy M.
Visible Genetics Inc.
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