High-density lipoprotein solid-base precipitation assay method

Details High-density lipoprotein solid-base precipitation assay method High-density lipoprotein solid-base precipitation assay method

1991-07-12

1993-05-25

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving cholesterol

435 25, 436169, 436170, C12Q 160, C12Q 126, G01N 2177

Patent

active

052139646

ABSTRACT:
An improved method for measuring the concentration of HDL-associated cholesterol in a blood-fluid sample. The method includes (a) adding a volume of the sample to an absorptive matrix (i) formed of glass fibers and (ii) effective to separate soluble from precipitated blood through the matrix from a sample-application site to a sample-collection site in the matrix, (b) releasing into the matrix, dextran sulfate and magnesium ions in an amount effective to selectively precipitate LDL and VLDL in the fluid sample, and (c) assaying fluid sample which has migrated through the matrix for the presence of lipoprotein-bound cholesterol. The improvement in the method comprises treating the glass fibers by reaction with bis(hydroxyethyl)aminopropyltriethoxy silane or by coating the fibers with polyvinyl alcohol, thereby affording substantially 100% recovery of HDL applied to the matrix.

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Bachorik, P. S., Methods in Enzymology (1986), vol. 129 "Precipitation Methods for Quantification of Lipoproteins" pp. 78-100, Academic Press, Inc.

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