High density lipoprotein cholesterol assay

Details High density lipoprotein cholesterol assay High density lipoprotein cholesterol assay

1978-09-27

1980-02-12

Marantz, Sidney

Chemistry: physical processes

Physical processes

Crystallization

252408, 435 11, G01N 3102, G01N 3316, G01N 3114

Patent

active

041881885

ABSTRACT:
A reagent composition useful in assays for high density lipoprotein (HDL) cholesterol comprises heparin and a salt of a divalent metal cation such as MnCl.sub.2 dispersed in an inert filler comprising a polysaccharide, a terminal interlocking linear glucose polymer and a vinylpyrrolidone. The cholesterol containing serum sample acts as the milieu of the reaction as well as the solvent for the reagent composition itself.

REFERENCES:
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W. T. Friedewald et al., Clin. Chem., 18(6), 499-502, (1972).
E. Cham, Clin. Chem., 22(11), 1812-1816, (1976).
Quanta-Zyme.RTM. Cholesterol Instruction Manual, Bio-Rad Laboratories, Bulletin 4219, May 1978.
HDL Cholesterol Instruction Manual, Bio-Rad Laboratories, 1978.

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