Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Means for analyzing liquid or solid sample
Patent
1995-05-23
1997-11-25
Elliott, George G.
Chemical apparatus and process disinfecting, deodorizing, preser
Analyzer, structured indicator, or manipulative laboratory...
Means for analyzing liquid or solid sample
422 8205, 422 8207, 422 8208, 422 8209, 2504581, 65409, 435 6, 435 912, G01N 1506, G01N 2129, C03B 3715, C12Q 168
Patent
active
056908946
ABSTRACT:
The invention relates to the fabrication and use of biosensors comprising a plurality of optical fibers each fiber having attached to its "sensor end" biological "binding partners" (molecules that specifically bind other molecules to form a binding complex such as antibody-antigen, lectin-carbohydrate, nucleic acid-nucleic acid, biotin-avidin, etc.). The biosensor preferably bears two or more different species of biological binding partner. The sensor is fabricated by providing a plurality of groups of optical fibers. Each group is treated as a batch to attach a different species of biological binding partner to the sensor ends of the fibers comprising that bundle. Each fiber, or group of fibers within a bundle, may be uniquely identified so that the fibers, or group of fibers, when later combined in an array of different fibers, can be discretely addressed. Fibers or groups of fibers are then selected and discretely separated from different bundles. The discretely separated fibers are then combined at their sensor ends to produce a high density sensor array of fibers capable of assaying simultaneously the binding of components of a test sample to the various binding partners on the different fibers of the sensor array. The transmission ends of the optical fibers are then discretely addressed to detectors--such as a multiplicity of optical sensors. An optical signal, produced by binding of the binding partner to its substrate to form a binding complex, is conducted through the optical fiber or group of fibers to a detector for each discrete test. By examining the addressed transmission ends of fibers, or groups of fibers, the addressed transmission ends can transmit unique patterns assisting in rapid sample identification by the sensor.
REFERENCES:
patent: H1398 (1995-01-01), Campbell
patent: 4447546 (1984-05-01), Hirschfeld
patent: 4582809 (1986-04-01), Block et al.
patent: 4909990 (1990-03-01), Block et al.
patent: 5061857 (1991-10-01), Thompson et al.
patent: 5082630 (1992-01-01), Partin et al.
patent: 5135876 (1992-08-01), Andrade et al.
patent: 5166990 (1992-11-01), Riccitelli et al.
patent: 5194393 (1993-03-01), Hugl et al.
patent: 5244636 (1993-09-01), Walt et al.
patent: 5250264 (1993-10-01), Walt et al.
patent: 5260029 (1993-11-01), Hosoi et al.
patent: 5304492 (1994-04-01), Klinkhammer
patent: 5344784 (1994-09-01), Attridge
patent: 5449625 (1995-09-01), Kobayashi et al.
Graham et al, "Gene probe assays on a fibre-optic evanescent wave biosensor", Biosens. Bioelect. 7:487-493, 1992.
Barnard et al, "A fibre optic chemical sensor with discrete sensing sites", Nature 353:338-340, Sep. 1991.
Lipson et al, "Multifiber, multiwavelength, fiber optic fluorescence spectrophotometer", IEEE Trans. Biom. Engineer. 39(9): 886-892, Sep. 1992.
Piunno et al, "Fiber optic DNA sensor for fluorometric nucleic acid determination", Anal. Chem. 67:2635-2643, Aug. 1995.
McCurley et al, "An optical biosensor using a fluorescent swelling sensing element", Biosens. Bioelectron. 9:527-533, 1994.
Anderson et al. Fiber optic based biosensor: signal enhancement in a production model. Proceedings SPIE--The International Society for Optical Engineering, vol. 1648: 39-43. Jan. 23-24, 1992.
Krug et al., Anal. Chim. Acta., 256(2): 263-268 (1992).
Blum et al. Anal. Chim. Acta., 226(2): 331-336 (1989).
Blum et al. Anal. Lett., 21(5): 717-726 (1988).
Hlavay et al. Biosensors and Bioelectronics, 9(3): 189-195 (1994).
Kallioniemi et al. Science, 258: 818-821 (1992).
Marx et al. J. Intelligent Material Systems and Structures, 5(4): 447-454 (1994).
Myrick et al. Fluorescence microstructure using a laser/fiber optic profiler . Proceedings of SPIE--The International Society for Optical Engineering, v 1302: 336-345 Apr. 16-18 (1990).
Gray Joe
Pinkel Daniel
Elliott George G.
Fredman Jeffrey
The Regents of the University of California
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