High copy-number plasmid vectors, production and use thereof

Details High copy-number plasmid vectors, production and use thereof High copy-number plasmid vectors, production and use thereof

1984-09-17

1987-10-27

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Vector, per se

435 91, 4351723, 435253, 935 39, 935 42, C12P 1934, C12N 1500, C12N 700, C12N 120

Patent

active

047030127

ABSTRACT:
Plasmid vectors and a process for the preparation thereof are disclosed having the same replication region as pBR322, but which have a substantially higher copy number per cell. The plasmid vectors contain a G.fwdarw.T point mutation at base pair 3074 of the pBR322 sequence which holds back synthesis of the repressor of plasmid replication, and also contain either no tetracycline resistance gene or the tetracycline resistance gene in an inactivated or weakened state.

REFERENCES:
Chem. Abstracts, vol. 91, No. 25, Dec. 17, 1979, 207243f.
Proc. Nat. Acad. Sci., vol. 79, pp. 3570-3574, Jun. 1982, Wong et al., Temperature-Sensitive Copy Number Mutants of ColEl are Located in an Untranslated Region of the Plasmid Genome.
Nature, vol. 283, Jan. 10, 1980, pp. 216-218, MacMillan Journals Ltd.; A. J. Twigg et al, Trans-Complementable Copy-number Mutants of Plasmid ColEl.
Molecular and General Genetics, vol. 188, 1982, pp. 338-344, M. J. J. Hakkaart et al., Maintenance of the Bacteriocinogenic Plasmid Clo DF13 in Escherichia Coli Cells.
Peden, K. W. C. Gene 22, pp. 277-280 (1983).
Stanier et al. in Microbiol World, 4th Ed. Prentice-Hall, Inc., 1976, pp. 413-414 and 441-444.
Lacatenu et al., Nature, 294: 623-626, 1981 (Dec.).
Donbora et al., P.N.A.S. USA, 78(8): 4699-4703, 1981 (Aug.).
Stougaard et al., P.N.A.S. USA, 78(10): 6008-6012, 1981 (Oct.).

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