High and low frequency analysis of osmotic stress of cells

Electricity: measuring and testing – Determining nonelectric properties by measuring electric...

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G01N 2700

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active

045352844

ABSTRACT:
Disclosed is a method wherein osmotic activity of biological cells immersed in a suspension solution is induced by an osmotic or lytic shock and is monitored in an electronic volume sensing particle analyzer of the Coulter Counter.RTM. type, the method including the steps of passing a high frequency current and a low frequency current through an aperture of said particle analyzer to create detectable signals for classifying the cells into populations of unaltered, altered, and ghost cells.

REFERENCES:
patent: 2656508 (1953-10-01), Coulter
patent: 3259842 (1966-07-01), Coulter et al.
patent: 3502973 (1970-03-01), Coulter et al.
patent: 3502974 (1970-03-01), Coulter et al.
patent: 3603875 (1971-09-01), Coulter et al.
patent: 3836849 (1974-09-01), Coulter et al.
patent: 4278936 (1981-07-01), Shine
patent: 4374644 (1983-02-01), Armstrong
"High Resolution Particle Analysis--Its Application to Platelet Counting and Suggestions for Further Application in Blood Cell Analysis", John L. Haynes, Blood Cells, 6, 201-213, 1980.
"Erythrocyte Osmotic Fragility: Micromethod Based on Resistive-Particle Counting", Adrian R. L. Gear, J. Lab. Clin. Med., pp. 914-928, Nov., 1977.

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