High affinity nucleic acid ligands containing modified nucleotid

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, 536 2431, 536 245, 536 231, 536 221, 935 77, 935 78, C12Q 168, C12P 1934, C07H 2102, C07H 2104

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059586915

ABSTRACT:
Nucleic acid ligands containing modified nucleotides are described as are methods for producing such oligonucleotides. Such ligands enrich the chemical diversity of the candidate mixture for the SELEX process. Specific examples are provided of nucleic acids containing nucleotides modified at the 2'- and 5-position. Specific 2-OH and 2'-NH.sub.2 modified RNA ligands to thrombin are described.

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