High-affinity choline transporter

Details High-affinity choline transporter High-affinity choline transporter

2000-08-18

2008-10-07

Chernyshev, Olga N. (Department: 1649)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S069700, C435S252300, C424S185100, C424S192100, C530S350000, C536S023100, C536S023400

Reexamination Certificate

active

07432077

ABSTRACT:
The present invention provides a protein having high-affinity choline transporter activity which is important physiologically, a gene encoding the protein, and a method of screening a material promoting the high-affinity choline transporter activity with the use of the same, and the like. By examining high-affinity choline uptake activity of Na+-dependent transporter cDNA deduced from the genomic sequence of a nematode (C. elegans) in aXenopusoocyte expression system, the cDNA (cho-1) of nematode high-affinity choline transporter is identified. Then the cDNA (CHT1) of rat high-affinity choline transporter is cloned from rat spinal cord by using the homology of a base sequence to this cDNA as an index. Similarly, the cDNA of human high-affinity choline transporter is cloned from human genome.

REFERENCES:
patent: 4946778 (1990-08-01), Ladner et al.
patent: 6287820 (2001-09-01), Umansky et al.
patent: 6500643 (2002-12-01), Wu et al.
patent: 2003/0022195 (2003-01-01), Curits
patent: 2003/0114399 (2003-06-01), Blakely et al.
Okuda et al. (Nov. 22, 2002) “Single nucleotide polymorphism of the human high affinity choline transporter alters transpo rate.” The Journal of Biological Chemistry 277(47): 45315-45322.
Wells (Sep. 18, 1990) “Additivity of Mutational Effects in Proteins.” Biochemistry 29(37): 8509-8517.
Ngo et al. (Mar. 2, 1995) “The Protein Folding Problem and Tertiary Structure Prediction, Chapter 14: Computational Complexity Protein Structure Prediction, and the Levinthal Paradox” pp. 492-495.
Bork (2000) “Powers and Pitfalls in Sequence Analysis: The 70% Hurdle.” Genome Research 10:398-400.
Skolnick and Fetrow (2000) “From gene to protein structure and function: novel applications of computational approaches in th genomic era.” Trends in Biotech. 18(1): 34-39.
Doerks et al., (Jun. 1998) “Protein annotation: detective work for function prediction.” Trends in Genetics 14(6): 248-250.
Smith and Zhang (Nov. 1997) “The challenges of genome sequence annotation or ‘The devil is in the details’.” Nature Biotechnology 15:1222-1223.
Brenner (Apr. 1999) “Errors in genome annotation.” Trends in Genetics 15(4): 132-133.
Bork and Bairoch (Oct. 1996) “Go hunting in sequence databases but watch out for the traps.” Trends in Genetics 12(10): 425-427.
Apparsundaram et al. (Oct. 5, 2000) “Molecular Cloning of a Human, Hemicholinium-3-Sensitive Choline Transporter.” Biochemical and Biophysical Research Communications 276(3): 862-867.
Slotkin et al. (Aug. 1994) “Overexpression of the High Affinity Choline Transporter in Cortical Regions Affected by Alzheimer Disease.” J. Clin. Invest. 94(2): 696-702.
Verma and Somia (Sep. 18, 1997) “Gene therapy- promises, problems, and prospects.” Nature 389: 239-241.
Kaneda (Sep. 2001) “Gene Therapy: A Battle Against Biological Barriers” Current Molecular Medicine 1(4): 493-499.
Eck and Wilson (1996) Chapter 5: “Gene-Based Therapy” Goodman & Gilmans's The Pharmacological Basis of Therapeutics 9th Ed. (pp. 77-100).
“Nucleic Acid Hybridization- General Aspects” pp. 33-37 Roche website retrieved on May 12, 2004.
NIH Division of Intramural Research “Nucleic Acid Hybridization” retrieved from NIH website on May 12, 2004.
Jane et al. (Oct. 1998) “Vector development: a major obstacle in human gene therapy.” Ann Med. 30(5): 413-5.
Potter & Chang (Jun. 18, 1999) “Review—The use of immunosuppressive agents to prevent neutralizing antibodies against a transgene product.” Ann N Y Acad Sci. 875: 159-74.
Rozmahel et al. (Jul. 1997) “Incomplete rescue of cystic fibrosis transmembrane conductance regulator deficient mice by the human CFTR cDNA.” Hum Mol Genet. 6(7): 1153-62.
Kandel et al. Principles of Neural Science (Box 45-1 pp. 890-895), 1999.
Busch et al. (Aug. 1998) “Human neurons express the polyspecific cation transporter hOCT2, which translocates monoamine neurotransmitters, amantadine, and memantine.” Mol Pharmacol. 54(2): 342-52.
Modrek et al. (2000). A genomic view of alternative splicing. Nature Genetics, 30, 13-19.
Kandel et al. Principles of Neural Science (Box 45-1 pp. 890-895), 1998.
Busch et al. (Aug. 1998) “Human neurons express the polyspecific cation transporter hOCT2, which translocates monoamine neurotransmitters, amantadine, and memantine.” Mol Pharmacol. 54(2): 342-52.
Davis et al., “Basic Methods In Molecular Biology”, Elsevier, pp. 1-3, (1986).
Haga, “Synthesis And Release Of [14C]Actylocholine In Synaptosomes”, Journal Of Neurochemistry, vol. 18, pp. 781-798, (1971).
“Choline: High-Affinity Uptake By Rat Brain Synaptosomes”, Science, vol. 178, pp. 626-628, (1972).
Haga et al., “Choline Uptake Systems Of Rat Brain Synaptosomes”, Biochimica et Biophysica Acta, Elsevier Scientific Publishing Company, vol. 291, pp. 564-575, (1973).
Guyenet et al., “Inhibition By Hemicholinium-3 of [14C] Acetylcholine Synthesis And [3H]Choline High-Affinity Uptake In Rat Striatal Synaptosomes” Molecular Pharmacology, Academic Press, Inc., vol. 9, pp. 630-639, (1973).
Barker et al., “Comparative Studies of Substrates And Inhibitors Of Choline Transport And Choline Acetyltransferase”, The Journal Of Pharmacology And Experimental Therapeutics, The Williams & Wilkins Co., vol. 192(1):86-94, (1975).
Kuhar et al., “Sodium-Dependent, High Affinity Choline Uptake”, Journal Of Neurochemistry, vol. 30, pp. 15-21, (1978).
Tucek, “Regulation Of Acetylcholine Synthesis In The Brain”, Journal Of Neurochemistry, International Society for Neurochemisty, vol. 44(1):11-24, (1985).
Happe et al., “High-Affinity Choline Transport Sites: Use of [3H]Hemicholinium-3 As A Quantitative Marker”, Journal of Neurochemistry, International Society For Neurochemistry, vol. 60(4):1191-1201, (1993).
Kuhar et al., “Choline: Selective Accumulation by Central Cholinergic Neurons”, Journal Of Neurochemistry, vol. 20, pp. 581-593, (1973).
Vickroy et al., “Reduced Density Of Sodium-Dependent [3H]Hemicholinium-3 Binding Sites In The Anterior Cerebral Cortex Of Rats Following Chemical Destruction Of The NucleusBasalis magnocellularis”, European Journal of Pharmacology, Elsevier Science Publishers B.V., vol. 102, pp. 369-370, (1984).
ELEGANS: Sequence to Biology, “Genome Sequence Of The NamatodeC. Elegans: Aplatform For Investigating Biology”, www.sciencemag.org, vol. 282, pp. 2012-2018, (1998).
Nelson, “The Family of Na+/C1-Neurotransmitter Transporters”, Journal of Neurochemistry, International Society For Neurochemistry, vol. 71(5):1785-1803, (1998).
Hediger et al., “Expression Cloning And cDNA Sequencing Of The Na+/Glucose Co-Transporter”, Nature, vol. 330, pp. 379-381, (1987).
Nikawa et al., “Primary Structure Of The Yeast Choline Transport Gene And Regulation Of Its Expression”, The Journal Of Biological Chemistry, The American Society for Biochemistry And Molecular Biology, Inc., vol. 265(26):15996-16003, (1990).
Schloss et al., “The Putative Rat Choline Transporter Choti Transports Creatine And Is Highly Expressed In Neural And Muscle-Rich Tissues”, Biochemical And Biophysical Research Communications, vol. 198(2):637-645, (1994).
Simon et al., “High Affinity Choline Uptake: Ionic And Energy Requirements”, Journal Of Neurochemistry, vol. 27, pp. 93-99, (1976).
Vickroy et al., “Sodium-Dependent Hogh-Affinity Binding of [3H]Hemicholonium-3 In the Rat Brain: A Potentially Selective Marker For presynaptic Cholinergic Sites”, Life Sciences, vol. 35, pp. 2335-2343, (1984).
Sandberg et al., “Characterization of [3H]Hemicholinium-3 Binding Associated With Neuronal Choline Uptake Sites In Rat Brain Membranes”, Brain Research,

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