High affinity, brain-specific nucleic acids encoding a L-proline

Details High affinity, brain-specific nucleic acids encoding a L-proline High affinity, brain-specific nucleic acids encoding a L-proline

1992-05-01

1996-12-03

Draper, Garnette D.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435 691, 4353201, 43525411, 4352523, 536 235, 536 2431, C12N 1512, C12N 510, C12N 119, C12N 121

Patent

active

055807755

ABSTRACT:
The anatomical distribution, nucleic acid sequence, pharmacological properties, and inferred structural features of a cDNA encoding a high affinity, Na.sup.+ -dependent rat brain L-proline transporter is described. The expression of this carrier in subpopulations of putative glutamatergic pathways supports a specific role for L-proline in excitatory amino acid neurotransmission. The cloned transporter cDNA predicts a 637 amino acid protein with 12 putative transmembrane domains and exhibits 44%-45% amino acid sequence identity with other neurotransmitter transporters. These findings support a synaptic role for L-proline in specific excitatory pathways in the CNS. The sequence can be used for expression of the transporter molecule, to make probes for the same protein from other species and related proteins, in diagnostic assays, and to design functional and structural analogs for use in research and possible clinical treatments. The protein is useful in making antibodies, conducting research studies, and design of therapeutic transporter modulators for clinical treatments.

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