HIFαprolyl hydroxylation assay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

Reexamination Certificate

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Details

C435S023000

Reexamination Certificate

active

07985563

ABSTRACT:
The present invention provides methods of identifying compounds that decrease proline hydroxylation of the alpha subunit of hypoxia inducible factor (HIFα).

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Epstein, Andrew C.R., et al., “C Elegans EGL-9 and Mammalian Homologs Define a Family of Dioxygenases that Regulate HIF by Prolyl Hydroxylation,” Cell (2001) 107:43-54.
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Ivan, Mircea, et al., “HIFα Targeted for VHL-Mediated Destruction by Proline Hydroxylation: Implications for O2 Sensing,” Science (2001) 292:464-468.
Jaakkola, Panu, et al., “Targeting of HIFα to the von Hippel-Lindau Ubiquitylation Complex by O2-Regulated Prolyl Hydroxylation,” Science (2001) 292:468-472.
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