Hexokinase obtained from thermophilic yeast Kluyveromyces fragil

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

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4352551, 424 9461, C12N 912, C12N 114, A61K 3847

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active

059486651

ABSTRACT:
The present invention relates to a hexokinase that is stable in solution whether in the presence or the absence of glucose. The present enzyme is obtained from a culture of the thermophilic yeast, Kluyveromyces. It can be used for determination of ATP and glucose, and for determination of a biological component which produces ATP or glucose (e.g. creatine kinase).

REFERENCES:
Garcia et al. "Caracterizacion de levaduras por enzimoforesis", Interferon y Biotechnologia (1985) 2(2): 129-136, 1985.
Hutchinson et al. "Hexokinase of the adult dog heartworm, Dirofilaria Immitis" Comp. Biochem. Physiol. (1977) 58B: 131-134, 1977.
I. Balesteros et al., "Optimization of the Simultaneous Saccharification and Fermentation Process Using Thermotolerant Yeasts.", Applied Biochemistry and Biotechnology, vol. 39/40, pp. 201-211 (1993).
Abstract of Japanese application No. 55-099190, published Jul. 28, 1980 from Derwent, accession No. 80-633830.
Abstract of Japanese application No. 05-111396, published May 7, 1993 from Derwent, accession No. 93-184816.
Clinical Chemistry, vol. 20 No. 4, pp. 247-254, 1991.
Clinical Chemistry, vol. 19 No. 2, pp. 185-208, 1990.

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