Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se
Patent
1996-01-19
1998-12-29
LeGuyader, John L.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Primate cell, per se
425 4, 425 6, 425 911, 425 9131, 425183, 4253201, 425325, 536 231, 536 2431, 536 245, C12N 1585, C12N 1500, C12N 1563, C12Q 168
Patent
active
058540679
ABSTRACT:
Disclosed are compositions and methods for inhibiting hexokinase enzymes in mammalian cells. Specifically provided are proteins that stimulate the production of trehalose-6-phosphate and their respective genes; hexokinase-specific ribozymes and genes encoding such constructs; and agents that competitively reduce hexokinase activity, e.g., by displacing hexokinase from mitochondria, and their respective genes. The latter group of agents includes inactive hexokinases and fragments thereof that retain mitochondrial binding functions and hexokinase-glucokinase chimeras that further substitute glucokinase activity for hexokinase activity. Mammalian cells including such hexokinase inhibitors, methods of making such cells and various in vitro and in vivo methods of using cells with reduced hexokinase activity are also described herein.
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Han He-Ping
Newgard Christopher B.
Normington Karl D.
Betagene, Inc.
Board of Regents , The University of Texas System
LeGuyader John L.
Wang Andrew
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