Heterotrophic algal high cell density production method and...

Chemistry: molecular biology and microbiology – Process of utilizing an enzyme or micro-organism to destroy... – Treating animal or plant material or micro-organism

Reexamination Certificate

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C435S257100, C514S558000

Reexamination Certificate

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07989195

ABSTRACT:
A multiphase culturing process for high density heterotrophic microalgal growth uses crude glycerol as the primary carbon source and produces ω-3 fatty acids. The process uses multiphase growth conditions that decouple the phases of increasing cell density and increasing cell size and fatty acid production. The entire process is integrated with biodiesel production.

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Yokochi et al., 1998, Applied Microbiology and Biotechnology, vol. 49, p. 72-76.
Wu et al., 2005, Process Biochemistry, vol. 40, p. 3103-3108.
Pyle, D.J., “Producing Docosahexaenoic Acid (DHA)-Rich Algae from Biodiesel-Derived Crude Glycerol: Effects of Impurities on DHA Production and Algal Biomass Composition.” Journal of Agricultural and Food Chemistry. 2008, 56, pp. 3933-3939.

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