Heteropolymeric triplexes containing watson-crick triplets

Details Heteropolymeric triplexes containing watson-crick triplets Heteropolymeric triplexes containing watson-crick triplets

2005-04-12

2005-04-12

Foreman, B J (Department: 1637)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S024300, C536S024310, C536S024320, C536S024330, C435S006120

Reexamination Certificate

active

06878815

ABSTRACT:
Triplex complexes contain a single-stranded probe bound to a double-stranded nucleic acid target, in which the probe includes a heteropolymeric nucleic acid or a heteropolymeric nucleic acid analog. All base triplets of the complex are members selected from the group consisting of A-T-A, T-A-T, U-A-T, T-A-U, A-U-A, U-A-U, G-C-G and C-G-C. A cation-facilitated assay includes detecting the presence of such triplex complexes to determine the degree of complementarity between the probe and target sequence. The assay preferably detects a change in fluorescent intensity of a label as a function of binding affinity between the probe and target. The label can be covalently tethered to the probe or to the target, or can be an intercalating fluorophore in the reaction medium.

REFERENCES:
patent: 4876187 (1989-10-01), Duck et al.
patent: 4963477 (1990-10-01), Tchen
patent: 5011769 (1991-04-01), Duck et al.
patent: 5403711 (1995-04-01), Walder et al.
patent: 5539082 (1996-07-01), Nielsen et al.
patent: 5558998 (1996-09-01), Hammond et al.
patent: 5660988 (1997-08-01), Duck et al.
patent: 5705346 (1998-01-01), Okamoto et al.
patent: 5707801 (1998-01-01), Bresser et al.
patent: 5731146 (1998-03-01), Duck et al.
patent: 5800984 (1998-09-01), Vary
patent: 5800992 (1998-09-01), Fodor et al.
patent: 5814516 (1998-09-01), Vo-Dinh
patent: 5824477 (1998-10-01), Stanley
patent: 5861124 (1999-01-01), Hosoi et al.
patent: 5888739 (1999-03-01), Pitner et al.
patent: 5912332 (1999-06-01), Agrawal et al.
patent: 5948897 (1999-09-01), Sen et al.
patent: 6013442 (2000-01-01), Kolesar et al.
patent: 6017709 (2000-01-01), Hardin et al.
patent: 6027880 (2000-02-01), Cronin et al.
patent: 6046004 (2000-04-01), Wu et al.
patent: 6048690 (2000-04-01), Heller et al.
patent: 6060242 (2000-05-01), Nie et al.
patent: 6107078 (2000-08-01), Keese et al.
patent: 6117657 (2000-09-01), Usman et al.
patent: 6251591 (2001-06-01), Wu et al.
patent: 6255050 (2001-07-01), Nie et al.
patent: 6255469 (2001-07-01), Seeman et al.
patent: 6265170 (2001-07-01), Picard et al.
patent: 6287772 (2001-09-01), Stefano et al.
patent: 6312925 (2001-11-01), Meyer, Jr. et al.
patent: 2333359 (1999-07-01), None
patent: WO 9745539 (1997-12-01), None
patent: 2338301 (1999-12-01), None
patent: WO 0020633 (2000-04-01), None
Baran et al.,Nucleic Acids Research25:297-303 (1997).
Bohmann et al.,Science, 238:1386-1392 (Dec. 1987).
Chan et al.,J. Mol. Med. 75 Issue 4:267-282 (1997).
Dalrymple et al.,Nucleic Acids Research, vol. 13, No. 21, pp. 7865-7879 (1985).
Hill et al.,Methods in Enzymology, 278:390-416 (1997).
Johansen and Jacobsen,J Biomol Struct Dyn, 16(2):205-22 (Oct. 1998) (Abstract).
Kadonaga et al.,Cell, 51:1079-1090 (Dec. 24, 1987).
Kukreti et al. 25Nucleic Acids Research4264-4270 (1997).
Marsh et al.,Nucleic Acids Research, 23:696-700 (1995).
Marsh et al.,Biochemistry33:10718-10724 (1994).
Mazumder et al.,Biochemistry35:13762-13771 (1996).
Sen et al.,Nature334:364-366 (Jul. 28, 1988).
Sen et al.,Biochemistry31:65-70 (1992).
Sturm et al.,Genes&Development, 2:1582-1599 (1988).
Watson, James, “A Personal Account of the Discovery of the Structure of DNA,” (1968).
Williamson et al.,Cell59:871-880 (Dec. 1, 1989).
Wilson et al.,Cell, 74:115-125 (Jul. 16, 1993).
Zhurkin et al.,J. Mol. Biol., vol. 239, 181-200 (1994).

Affiliated with

Also associated with

Rating

Heteropolymeric triplexes containing watson-crick triplets does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Heteropolymeric triplexes containing watson-crick triplets, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Heteropolymeric triplexes containing watson-crick triplets will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3437509