Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1995-06-07
1999-12-21
LeGuyader, John L.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 691, 4351723, 435183, 43525411, 4352543, 4352546, 536 231, 536 232, C12N 115, C12N 1580
Patent
active
060047852
ABSTRACT:
Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence. Preferably functional polyadenylation sequences are operably linked to the 3' terminus of the DNA sequence encoding the heterologous polypeptides. Each of the above described vectors are used in novel processes to transform a filamentous fungus wherein the DNA sequences encoding the signal sequence and heterologous polypeptide are expressed. The thus synthesized polypeptide is thereafter secreted from the filamentous fungus.
REFERENCES:
patent: 4486533 (1984-12-01), Lambowitz
patent: 4492758 (1985-01-01), Esser et al.
patent: 4666847 (1987-05-01), Alford et al.
patent: 4794175 (1988-12-01), Nunberg et al.
patent: 4816405 (1989-03-01), Yelton et al.
patent: 4863864 (1989-09-01), Ashikari et al.
patent: 5198345 (1993-03-01), Gwynne et al.
Stohl, L. L., et al., Construction of a shuttle vector for the filamentous fungus Neurospora crassa, Proc. Natl. Acad. Sci. USA, 80:1058-1062 (1983).
Ballance, D. J., et al., "Transformation of Aspergillus Nidulans By The Orotidine-5'-Phosphate Decarboxylase Gene", Biochemical and Biophysical Research Communications, 112(1):284-289 (1983).
Tilburn, J., et al., "Transformation by integration in Aspergillus nidulans", Gene, 26:205-221 (1983).
Yelton, M. M., et al., "Transformation of Aspergillus nidulans by using a trpC plasmid", Proc. Natl. Acad. Sci. USA, 81:1470-1474 (1984).
Kuck, U., et al., "II. Recombination: a) DNA Transformation in Cyanobacteria, Algae and Fungi:Trends and Perspectives", Progress in Botany, 46:201-204 (1984).
Krieg et al., J. Mol. Biol., 180:615-640 (1984).
Warren et al., Cell, 39 (2):547-555 (1984).
Innis et al., (1985) Science, vol. 228, pp. 21-26.
Boel et al., EMBO J., 3(5):1097-1102 (May 1984).
Mellor et al. Gene, 24:1-14 (Sep. 1983).
Stohl, L. L., et al. "Characterization of deletion derivatives of an autonomously replicating Neurospora plasmid", Nucleic Acids Research, 12:6169-6178 (1984).
Grant, D. M., et al., "Transformation of Neurospora crassa with Recombinant Plasmids Containing the Cloned Glutamate Dehydrogenase (am) Gene:Evidence for Autonomous Replication of the Transforming Plasmid", Molecular and Cellular Biology, 4(10):2041-2051 (1984).
Buxton, F. P., et al., "The transformation of mycelial spheroplasts of Neurospora crassa and the attempted isolation of an autonomous replicator", Mol. Gen. Genet, 196:339-344 (1984).
Kinsey, J. A., et al., "Transformation of Neurospora crassa with the Cloned am (Glutamate Dehydrogenase) Gene", Molecular and Cellular Biology, 4(1):117-122 (1984).
Bull, J. H., et al., Heavily methylated amplified DNA in transformants of Neurospora crassa, Nature, 310:701-704 (1984).
Miller, B. L., et al., "Direct and Indirect Gene Replacements in Aspergillus nidulans", Molecular and Cellular Biology, 5(7):1714-1721 (1985).
Weiss, R. L., et al., Expression of Aspergillus Genes in Neurospora, Gene Manipulation in Fungi, (1985).
Johnstone, I. L., "Transformation of Aspergillus nidulans", Microbiological Sciences, 2(10):307-311 (1985).
Johnstone, I. L., et al., "Cloning an Aspergillus nidulans developmental gene by transformation", The EMBO Journal, 4(5):1307-1311 (1985).
Buxton, F. P., et al., "Transformation of Aspergillus niger using the argB gene of Aspergillus nidulans", Gene, 37:207-214 (1985).
Turner, G., et al., "Cloning and Transformation in Aspergillus", Gene Manipulations in Fungi, (1985).
Ballance, D. J., et al., "Development of a high-frequency transforming vector for Aspergillus nidulans", Gene, 36:321-331 (1985).
Yelton, M. M., et al., "A cosmid for selecting genes by complementation in Aspergillus nidulans:Section of the developmentally regulared yA locus", Proc. Natl. Acad. Sci. USA, 82:834-838 (1985).
Kelly, J. M., et al., "Transformation of Aspergillus niger by the amdS gene of Aspergillus nidulans", The EMBO Journal, 4(2):475-479 (1985).
Akins, R. A., et al., "General Method for Cloning Neurospora crassa Nuclear GEnes by Complementation of Mutants", Molecular and Cellular Biology, 5(9):2272-2278 (1985).
Paietta, J., et al., "Plasmid recovery from transformants and the isolation of chromosomal DNA segments improving plasmid replication in Neurospora crassa", Current Genetics, 9:383-388 (1985).
Kuiper, M. T. R., et al., "A recombinant plasmid carrying the mitochondrial plasmid sequence of Neurospora intermedia LaBelle yields new plasmid derivatives in Neurospora crassa transformants", Current Genetics, 9:471-477 (1985).
Dhawale, S. S., et al., "Transformation of Neurospora crassa with circular and linear DNA and analysis of the fate of the transforming DNA", Current Genetics, 10:205-212 (1985).
Wernars, K., et al., "Gene amplification in Aspergillus nidulans by transformation with vectors containing the amdS gene", Current Genetics, 9:361-368 (1985).
Saunders, G., et al., "Fungal cloning vectors", Trends in Biotechnology, 4:93-98 (1986).
Hynes, M. J., "Topical Review--Transformation of Filamentous Fungi", Experimental Mycology, 10:1-8 (1986).
Durrens, P., et al., "Heterologous insertion of transforming DNA and generation of new deletions associated with transformation in Aspergillus nidulans", Mol. Gen. Genet., 203:544-549 (1986).
Barnes, D. E., et al., "Behaviour of recombinant plasmids in Aspergillus nidulans:structure and stability", Current Genetics, 10:767-775 (1986).
Upshall, A., "Genetic and molecular characterization of argB+ transformants of Aspergillus nidulans", Current Genetics, 10:593-599 (1986).
Viebrock, A., et al., "The imported preprotein of the proteolipid subunit of the mitochondrial ATP synthase from Neurospora crassa. Molecular cloning and sequencing of the mRNA", The EMBO Journal, 1(5):565-571 (1982).
Alton, N. K., et al., "5'-Untranslated sequences of two structural genes in the qa gene cluster of Neurospora crassa", Proc. Natl. Acad. Sci. USA, 79:1655-1959 (1982).
Keesey, J. K., Jr., et al., "Cloning of the trp-1 Gene from Neurospora crassa by Complementation of a trpC Mutation in Escherichia coli", Journal of Bacteriology, 152(2):954-958 (1982).
Shoemaker, S., et al., Molecular Cloning of Exo-Cellobiohyrdolase I Derived From Trichoderma Reesei Strain L27, Biotechnology, 1:691-697 (1983).
Kinnaird, J. H., et al., "The complete nucleotide sequence of the Neurospora crassa am (NADP-specific glutamate dehydrogenase) gene", Gene, 26:253-260 (1983).
Berse, B., et al., "Cloning and characterization of the ornithine carbamoyltransferase gene from Aspergillus nidulans", Gene, 25:109-117 (1983).
Buxton, F. P., et al., "Cloning of the Structural Gene for Orotidine 5'-Phosphate Carboxylase of Neurospora crassa by Expression in Escherichia coli", Mol. Gen. Genet, 190:403-405 (1983).
Harris et al., Nucleic Acids Res., 10(7):2177-2187 (Apr. 1982).
Woudt, L. P., et al., ".sub.-- ", Nucleic Acids Research, 11:5353-6346 (1983).
Hynes, M. J., et al., "Isolation of Genomic Clones Containing the amdS Gene of Aspergillus nidulans and Their Use in the Analysis of Structural and Regulatory Mutations", Molecular and Cellular Biology, 3(8):1430-1439 (1983).
Arends, H., et al., "Nucleotide sequence of the cloned mRNA and gene of the ADP/ATP carrier from Neurospora crassa", The EMBO Journal, 3(2):377-382 (1984).
Nunberg, J. H., et al., Molecular Cloning and Characterization of
Berka Randy Michael
Cullen Daniel
Gray Gregory Lawrence
Hayenga Kirk James
Lawlis Virgil Bryan
Genencor International Inc.
LeGuyader John L.
Test Flehr Hohbach
Trecartin Richard F.
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