Heterologous gene expression with poliovirus replicon

Details Heterologous gene expression with poliovirus replicon Heterologous gene expression with poliovirus replicon

1998-09-02

2000-12-05

Mosher, Mary E.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

536 2372, 536 241, 4353201, 435353, 435358, 435364, 435367, 435371, 435455, C12N 1563, C12N 1586, C12N 510, C12P 2100

Patent

active

061565385

ABSTRACT:
The replication machinery of polio virus is used to express heterologous gene products, such as chloramphenicol acetyl transferase, in mammalian cells. Detectable expression following DNA transfection demonstrated that a polio replicon containing a foreign gene in the P1 region transcribed from an inducible promoter can be exported from the nucleus to the cytoplasm. The proteins in the P2/P3 region of the RNA can be translated and thereby render the RNA capable of replication. A stable cell line harbouring the polio replicon in the genome results in constitutive expression of chloramphenicol acetyl transferase or other heterologous gene product.

REFERENCES:
patent: 5789245 (1998-08-01), Dubensky et al.
Mattion et al, Journal of Virology, 68(6): 3925-3933, Jun. 1994.
Alexander et al, AIDS Research and Human Retroviruses, 10 (sup. 2): S57-S60, 1994.
Anderson et al, Virology 219: 140-149, 1996.
Filmus et al, Nucleic Acids Research, 20(11): 2755-2760, 1992.

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