Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide contains a tissue – organ – or cell...
Patent
1995-06-07
2000-02-29
McElwain, Elizabeth F.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide contains a tissue, organ, or cell...
800298, 435 691, 4353201, 435419, 435468, 536 241, A01H 500, C12N 1582, C07H 2104
Patent
active
060311568
ABSTRACT:
The identification and cloning of the gene(s) for plant asparagine synthetase (AS), an important enzyme involved in the formation of asparagine, a major nitrogen transport compound of higher-plants is described. Expression vectors constructed with the AS coding-sequence may be utilized to produce plant AS; to engineer herbicide resistant plants, salt/drought tolerant plants or pathogen resistant plants; as a dominant selectable marker; or to select for novel herbicides or compounds useful as agents that synchronize plant cells in culture.
The promoter for plant AS, which directs high levels of gene expression and is induced in an organ specific manner and by darkness, is also described. The AS promoter may be used to direct the expression of heterologous coding sequences in appropriate hosts.
REFERENCES:
patent: 4771002 (1988-09-01), Gelvin
patent: 4935363 (1990-06-01), Brown et al.
patent: 4962028 (1990-10-01), Bedbrook et al.
Andrulis et al., 1985, "Characterization of Single Step Albizziin-resistant Chinese Hamster Ovary Cell Lines with Elevated Levels of Asparagine Synthetase Activity", J. Biol. Chem. 260:7523-7527.
Andrulis et al., 1987, "Isolation of Human cDNAs for Asparagine Synthetase and Expression in Jensen Rat Sarcoma Cells", Mol. & Cell. Biol. 7:2435-2443.
Arfin et al., 1983, "Assignment of Structural Gene for Asparagine Synthetase to Human Chromosome 7", Somatic Cell Genet. 9:517-531.
Cartier et al., 1987, "Use of the Escherichia coli Gene for Asparagine Synthetase as a Selective Marker in a Shuttle Vector Capable of Dominant Transfection and Amplification in Animal Cells", Mol. & Cell. Biol. 7:1623-1628.
Davidson & Niswander, 1983, "Partial cDNA sequence to a hamster gene corrects defect in Escherichia coli pyrB mutant", Proc. Nat'l Acad. Sci. USA 80:6897-6901.
Herrera-Estrella et al., 1980, "Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector", Nature 310:115-120.
Hongo et al., 1978, "Purification and Properties of Asparagine Synthetase from Rat Liver", Biochim.& Biophys. Acta. 522:258-266.
Hongo & Sato, 1981, "Purification of Rat Liver Aspargine Synthetase by Affinity Chromatography on Reactive Blue 2-Agarose", Anal. Biochem. 114:163-166.
Hongo & Sato, 1983, "Some Molecular Properties of Asparagine Synthetase from Rat Liver", Biochim. & Biophys. Acta. 742:484-489.
Huber & Streeter, 1984, "Asparagine Biosynthesis in Soybean Nodules", Plant Physiol. 74:605-610.
Huber & Streeter, 1985, "Purification and Properties of Asparagine Synthetase from Soybean Root Nodules", Plant Science 42:09-17.
Humbert & Simoni, 1980, "Genetic and Biochemical Studies Demonstrating a Second Gene Coding for Asparagine Synthetase in Escherichia coli", J. Bacteriol. 142:212-220.
Jones, 1978, "L-Asparagine Auxotrophs of Saccharomyces cerevisiae: Genetic and Phenotypic Characterization", J. Bacteriol. 134:200-207.
Joy et al., 1983, "Asparagine Synthesis in Pea Leaves, and the Occurrence of an Asparagine Synthetase Inhibitor", Plant Physiology 73:165-168.
Lea & Fowden, 1975, "The purification and properties of glutamine-dependent asparagine synthetase isolated from Lupinus albus", Proc. R. Soc. Lond. B. 192:13-26.
Loyola-Vargas et al., 1988, "Nitrogen Metabolism in Canavalia ensiformis(L.)DC.II. Changing Activities of Nitrogen-Assimilating Enzymes during Growth", J. Plant Physiol. 132:289-293.
Maniatis et al., 1982, "Molecular Cloning A Laboratory Manual", Cold Spring Harbor Laboratory pp. 295-299.
Markin & Schuster, 1979, "Multiple Forms of Rat Liver L-Asparagine Synthetase", Biochem. & Biophys. Research Communications 88:583-588.
Milman & Cooney, 1979, "Partial Purification and Properties of L-Asparagine Synthetase from Mouse Pancreas", Biochem. J. 181:51-59.
Murray et al., 1991, "Analysis of unstable RNA transcripts of insecticidal crystal protein genes of Bacillus thuringiensis in transgenic plants and electroporated protoplasts", Plant Molecular Biol. 16:1035-1050.
Nakamura et al., 1981, "Nucleotide sequence of the asnA gene coding for asparagine synthetase of E. coli K-12", Nuc. Acid Res. 9:4669-4676.
Perlak et al., 1991, "Modification of the coding sequence enhances plant expression of insect control protein genes", Proc. Nat'l. Acad. Sci. USA88:3324-3328.
Pfeiffer et al., 1986, "Monoclonal Antibodies Specific for Bovine Pancreatic Asparagine Synthetase", J. of Biol. Chem. 261:1914-1919.
Pfeiffer et al., 1987, "Topographical Separation of the Catalytic Sites of Asparagine Synthetase Explored with Monoclonal Antibodies", J. of Biol. Chem. 262:11565-11570.
Pharmacia, 1989, "Cloning Vectors", Pharmacia LKB Biotechnology Products Catalog.
Ramnefjell & Rognes, 1981, "Asparagine Synthetase from Wheat Seedlings", Suppl. Plant Physiol. pp. 45, Abstr. 244.
Ramos & Wiame, 1980, "Two Asparagine Synthetases in Saccharomyces cerevisiae", Eur. J. Biochem. 108:373-377.
Ray et al., 1984, "Molecular cloning of a cDNA for Chinese hamster ovary asparagine synthetase", Gene 30:1-9.
Rognes, 1975, "Glutamine-Dependent Asparagine Synthetase from Lupinus luteus", Phytochemistry 14:1975-1982.
Roth & Lark, 1984, "Isopropyl-N(3-chlorophenyl)carbamate (CIPC) induced chromosomal loss in soybean: a new tool for plant somatic cell genetics", Theor. Appl. Genet. 68:421-431.
Scofield & Schuster, 1988, "The E. coli Asparagine Synthetase B Gene", J. Cell Biology 107:535a, Abstr. 3023.
Shelp & Atkins, 1984, "Subcellular Location of Enzymes of Ammonia Assimilation and Asparagine Synthesis in Root Nodules of Lupinus albus L.", Plant Science Letters 36:225-230.
Smith et al., 1984, "Assignment of the gene for asparagine synthetase to chromosome 7", Cytogen. Cell Genet. 37:1985-1986.
Tingey et al., 1987, "Glutamine synthetase genes of pea encode distinct polypeptides which are differentially expressed in leaves, roots and nodules", EMBO Journal 6:1-9.
Tsai & Coruzzi, 1990, "Dark-induced and organ-specific expression of two asparagine synthetase genes in Pisum sativum", EMBO Journal 9:323-332.
Van Heeke & Schuster, 1989, "Expression of Human Asparagine Synthetase in Escherichia coli", J. of Biol. Chem. 264:5503-5509.
Waye & Stanners, 1983, "Direct Transfer of the Bacterial Asparagine Synthetase Gene to Mammalian Cells", J. of Mol. & Appl. Genetics 2:69-82.
Wilson, 1971, "Botany", Holt, Rinehart & Winston, N.Y. pp. 447-451.
Kim et al. (1994) Plant Molec. Biol 24: 105-117.
Coruzzi Gloria M.
Tsai Fong-Ying
McElwain Elizabeth F.
New York University
LandOfFree
Heterologous gene expression utilizing plant asparagine syntheta does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Heterologous gene expression utilizing plant asparagine syntheta, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Heterologous gene expression utilizing plant asparagine syntheta will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-684690