Heterologous expression of an Aspergillus kawachi...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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C435S203000, C435S205000

Reexamination Certificate

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07037704

ABSTRACT:
The present invention relates to a granular starch hydrolyzing enzyme composition comprising an acid stable alpha amylase (asAA) having granular starch hydrolyzing activity. The invention also relates to a one-step method for producing an alcohol which comprises contacting a granular starch substrate with an acid-stable alpha amylase (asAA) having granular starch hydrolyzing (GSH) activity and a glucoamylase (GA) in a fermentation step which also comprises ethanologenic microorganisms at a temperature of 25–40° C. to obtain a fermentation broth having 5 to 20% ethanol.

REFERENCES:
Kajiwara, Y. et al., Production of Acid-Stable α-Amylase byAspergillus kawachiiduring BarleyShochu-KojiProduction,Journal of Fermentation and Bioengineering, 84(3):224-227, 1997.
Copy of the International Search Report for PCT/US04/41276, filed Dec. 9, 2004.

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