Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1994-02-03
1997-07-01
Fleisher, Mindy
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4352543, 435 694, C12P 2104, C12N 115
Patent
active
056437455
ABSTRACT:
The invention relates to a heterokaryotic filamentous fungus host capable of producing a heterologous heterodimer comprising at least two subunits. The heterokaryon contains a first and second nucleus; each nucleus contains an expression system for one subunit of the heterodimer. The heterokaryon is prepared by culturing together a first fungus host strain and a second fungus host strain that is homozygous with the first fungus host strain with respect to all heterokaryon compatibility alleles, wherein the first and second fungus host strains are cultured together under conditions wherein neither the first nor the second fungus strain can survive unless the heterokaryotic host is formed.
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Fleisher Mindy
Ketter James
University of Hawai'i
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