Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus
Patent
1997-11-25
2000-03-14
Housel, James C.
Drug, bio-affecting and body treating compositions
Whole live micro-organism, cell, or virus containing
Genetically modified micro-organism, cell, or virus
424 931, 424 934, 424 9341, 4241921, 424 934, 4242341, 4242361, 4242611, 4242391, 4242471, 514837, A01N 6300, A61K 3900, A61K 3902, A61K 3908
Patent
active
060369531
ABSTRACT:
Disclosed is a V. cholerae cell containing a DNA encoding a fusion polypeptide that includes a heterologous antigenic polypeptide sequence such as a portion of C. difficile toxin A. The fusion polypeptide also includes one of the following: (i) an E. coli hemolysin A subunit (wich is coexpressed with sequences encoding hemolysin B and D subunits); (ii) a secretion signal sequence and cholera toxin A2 subunit (which is coexpressed with cholera toxin B subunit); or (iii) cholera toxin B subunit.
REFERENCES:
patent: 4882278 (1989-11-01), Mekalanos
patent: 5143830 (1992-09-01), Holland et al.
patent: 5747028 (1998-05-01), Calderwood et al.
U.S. application No. 08/403,584, Mekalanos et al, filed Mar. 14, 1996.
Gentschev et al. Infect. Immun. 60: 5091-5098, 1992.
Abstracts of the 89th Annual Meeting of the American Society for Microbiology, p. 128, 1989.
Forrest et al., Immunogenicity of a Candidate Live Oral Typhoid/Cholera Hybrid Vaccine in Humans, J. Infectious Diseases 159:145:146, 1989.
Miller and Mekalanos, Strategies for the Development of Vaccines for Typhoid Fever, Shigellosis, and Cholera, Biomedical Science and the Third World 569:145-154, 1989.
Nashar et al., Current Progress in the Development of the B Subunits of Cholera Toxin and Escherichia coli Heat-labile Enterotoxin . . . the Oral Delivery of Heterologous Antigens and Epitopes, Vaccine 11:235-240, 1993.
Acheson et al., Protective Immunity to Shiga-Like Toxin I Following Oral Immunization with Shiga-Like Toxin I B-Subunit-Producing Vibrio cholerae CVD 103-HgR, Infection and Immunity 64:355-357, 1996.
Backstrom et al., Insertion of a HIV-1-Neutralizing Epitope in a Surface-Exposed Internal Region of the Cholera toxin B-subunit, Gene p. 211-217, 1994.
Jobling et al., Fusion Proteins Containing the A2 Domain of Cholera Toxin Assemble with B Polypeptides of Cholera Toxin to Form Immunoreactive . . . Holotoxin-Like Chimeras, Infection & Immunity 60:4915-4924, 1992.
Kaper and Levine, Recombinant Attenuated Vibrio Cholerae Stains Used as Live Oral Vaccines, Res. Microbiol. 141:901-906, 1990.
Kaper et al., A Recombinant Live Oral Cholera Vaccine, Bio/Technology 345-349, Apr. 1984.
Pierce et al., Determinants of the Immunogenicity of Live Virulent and Mutant Vibrio cholerae 01 in Rabbit Intestine, Infection and Immunity 55:477-481, 1987.
Sanchez et al., Genetic Fusion of a Non-toxic Heat-Stable Enterotoxin-Related Decapeptide Antigen to Cholera Toxin B-subunit, FEBS Letters 241:110-114, 1988.
Sanchez et al., Recombinant System for Overexpression of Cholera Toxin B Subunit in Vibrio cholerae as a Basis for Vaccine Development, Proc. Natl. Acad. Sci. USA 86:481-485, 1989.
Schodel et al., Synthesis in Vibrio cholerae and Secretion of Hepatitis B Virus Antigens Fused to Escherichia coli Heat-labile Exterotoxin Subunit B, Gene 255-259, 1991.
Tacket et al., Cholera Vaccines, Chapter 3.
Acheson et al., "Comparison of shiga-like toxin I B-subunit expression and Localization in Escherichia coli and vibrio cholerae by using trc or iron-regulated promoter systems", Infect. & Immun. 61:1098-1104 (1993).
Mobassaleh et al., "Pathogenesis of shigella diarrhea: evidence for a developmentally regulated glycolipid receptor for shigella toxin involved in the fluid secretory response of rabbit small intestine", J. Infect. Dis. 157:1023-1031 (1988).
Cray et al., "Successful colonization and immunization of adult rabbits by oral inoculation with vibrio cholerae 01", Infect. and Immun. 41:735-741 (1983).
Mekalanos et al., Cholera Toxin Genes: Nucleotide Sequence, Deletion Analysis and Vaccine Developemnt, Nature 306:551-557, 1983.
Kaper et al., Recombinant Nontoxinogenic vibrio Cholerae Strains as Attenuated Cholera Vaccine Candidates, Nature 308:655-658, 1984.
Levine et al., Safety, Immunogenicity, and Efficacy of Recombinant Live Oral Cholera Vaccines, CVD 103 and CVD 103-HgR, Lancet 468-470, 1988.
Levine et al., Volunteer Studies of Deletion Mutants of vibrio Cholerae 01 Prepared by Recombinant Techniques, Infec. Immun. 56:161-167, 1988.
Herrington et al., Toxin, Toxin-Coregulated Pili, and the toxR Regulon are Essential for Vibrio Chlerae Pathogenesis in Humans, J. Exp. Med. 168:1487-1492, 1988.
Goldberg et al., Identification of an Iron-Regulated Virulence Determinant in Vibrio Cholerae, Using TnphoA Mutagenesis, Infec. Immun. 58:55-60, 1990.
Taylor et al., Use of phoA Gene Fusions to Identify a Pilus Colonization Factor Coordinately Regulated with Cholera Toxin, Proc. Natl. Acad. Sci. USA 84:2833-2837, 1987.
Taylor et al., Broad-Host-Range Vectors for Delivery of TnphoA: Use in Genetic Analysis of Secreted Virulence Determinants of Vibrio Cholerae, J. Bac. 171:1870-1878, 1989.
Carniel et al., Molecular Cloning, Iron-Regulation and Mutagenesis of the irp2 Gene encoding HMWP2, a Protein Specific for the Highly Pathogenic Yersinia, Mol. Micro. 6:379-388, 1991.
Keren et al., Direct Demonstration in Intestinal Secretions of an IgA Memory Response to Orally Administered Shigella Flexneri Antigens, J. Immun. 128:475-479, 1982.
Bjorn et al., Effect of Iron on Yields of Exotoxin A in Cultures of Pseudomonas Aeruginosa PA-103, Infec. Immun. 19:785-791, 1978.
Black et al., Protective Efficacy in Humans of Killed Whole-Vibrio Oral Cholera Vaccine with and without the B Submit of Cholera Toxin, Infec. Immun. 55:1116-1120, 1987.
Blomfield et al., Allelic Exchange in Escherichia coli Using the Bacillus Subtilis sacB Gene and a Temperature-Sensitive pSC101 Replicon, Mol. Micro. 5:1447-1457, 1991.
Calderwood et al., Nucleotide Sequence of the Shiga-Like Toxin Genes of Escherichia coli, Proc. Natl. Acad. Sci. USA 84:4364-4368, 1987.
Calderwood et al., A System for Production and Rapid Purification of Large Amounts of the Shiga Toxin/Shiga-Like Toxin I B Subunit, Infec. Immun. 58:2977-2982, 1990.
Calderwood and Mekalanos, Iron Regulation of Shiga-Like Toxin Expression in Escherichia Coli Is Mediated By the Fur Locus, J. Bac. 169:4759-4764, 1987.
Clemens et al., Impact of B Subunit Killed Whole-Cell and Killed Whole-Cell-Only Oral Vaccines . . . Illness and Mortality in an Area Endemic for Cholera, Lancet 1:375-1379, 1988.
Czerkinsky et al., Antibody-Producing Cells in Peripheral Blood and Salivary Glands after Oral Cholera Vaccination of Humans, Infec. Immun. 59:996-1001, 1991.
Daskaleros et al., Iron Uptake in Plesiomonas Shigelloides: Cloning of the Genes for the Heme-Iron Uptake System, Infec. Immun. 59:2706-2711, 1991.
De Grandis et al., Nucleotide Sequence and Promoter Mapping of the Escherichia Coli Shiga-Like Toxin Operon of Bacteriophage H-19B, J. Bac. 169:4313-4319, 1987.
De Lorenzo et al., Operator Sequences of the Aerobactin Operon of Plasmid ColV-K30 Binding the Ferric Uptake Regulation (fur) Repressor, J. Bac. 169:2624-2630, 1987.
Donnenberg and Kaper et al., construction of an eae Deletion Mutant of Enteropathogenic Escherichia Coli By Using a Positive-Selection Suicide Vector, Infec. Immun. 59:4310-4317, 1991.
Donohue-Rolfe et al., Pathogenesis of Shigella Diarrhea, J. Exp. Med. 160:1767-1781, 1984.
Donohue-Rolfe et al., Enzyme-Linked Immunosorbent Assay for Shigella Toxin, J. Clin. Micro. 24:65-68, 1986.
Fernandez-Beros et al., Immune Response to the Iron-Deprivation-Induced Proteins of Salmonella Typhi in Typhoid Fever, Infec. Immun. 57:1271-1275, 1989.
Gentry et al., Quantitative Microtiter Cytotoxicity Assay for Shigella Toxin, J. Clin. Micro. 12:361-366, 1980.
Goldberg et al., Positive Transcriptional Regulation of an Iron-Regulated Virulence Gene in Vibrio Cholerae, Proc. Natl. Acad. Sci. USA 88:1125-1129, 1991.
Goldberg et al., Transcriptional Regulation by Iron of a Vibrio Cholerae Virulence Gene and Homology of the Escherichia Coli Fur System, J. Bac. 172:6863-6870, 1990.
Goldberg et al., Characterization of a Vibrio Cholerae Virulence Factor Homologous to the Family of TonB-Dependent Proteins, Mol. Micro. 6:2407-2418, 1992.
Harari et al., Synthetic Peptides of Shiga Toxin B Subunit Induce Antibodies Which Neutralize its Biological Activity, Infec. Immun. 56:1618-1624, 1988.
Harari and Arnon et al., Carboxy-Terminal Peptides from the
Calderwood Stephen B.
Ryan Edward T.
Devi S.
Housel James C.
The General Hospital Corporation
LandOfFree
Heterologous antigens in live cell V. cholerae strains does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Heterologous antigens in live cell V. cholerae strains, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Heterologous antigens in live cell V. cholerae strains will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-166423