Heterohybridomas producing human monoclonal antibodies to HIV-1

Details Heterohybridomas producing human monoclonal antibodies to HIV-1 Heterohybridomas producing human monoclonal antibodies to HIV-1

1994-11-21

1999-06-22

Budens, Robert D.

Drug, bio-affecting and body treating compositions

Immunoglobulin, antiserum, antibody, or antibody fragment,...

Structurally-modified antibody, immunoglobulin, or fragment...

4241421, 4241481, 4241601, 435 696, 435 7021, 4351722, 435328, 4353391, 5303873, 53038815, 53038835, A61K 39395, C07K 1610

Patent

active

059141093

ABSTRACT:
Heterohybridomas producing human monoclonal antibodies against a neutralizing epitope of HIV-1 are prepared by transforming peripheral blood lymphocytes by Epstein-Barr virus, selecting cells producing an antibody to a synthetic peptide representing part of the neutralizing epitope, and fusing them with a heteromyeloma cell. A method for producing a human monoclonal antibody specific for a neutralizing HIV-1 epitope from the heterohybridoma is disclosed. Also provided are human neutralizing monoclonal antibodies specific for particular peptide epitopes of the V3 loop of the gp120 glycoprotein of HIV, in particular a broadly reactive antibody specific for diverse HIV-1 strains, preferably recognizing an epitope comprising an amino acid sequence GPXR or GRAF.

REFERENCES:
patent: 4725669 (1988-02-01), Essex et al.
Fahey et al., "Status of Immune Based Therapies in HIV Infection and AIDS," Clin. Exp. Immunol. 88:1-5, Apr. 1992.
Fox, J.L., "No Winners Against AIDS," Biol Technology 12:128, Feb. 1994.
Casali et al., Science 234:476-9, 1986.
Rudikoff et al PNAS USA 79:1979-83 1982.
Morrison Science 229:1202-1207, 1985.
Gorny et al. PNAS USA 86:1624-1628 Mar. 1989.
Javaherian et al. Science 250:1590-1593, 1990.
Teng et al. PNAS USA 80:7308-7312, 1983.
Goudsmit et al., AIDS 2(3):157-164 1988.
Robinson et al, AIDS Res Hum Retroviruses 6(5):567-79 May 1990.
Karwowska et al. in Vaccines 92, Brown et al. Eds. CSHL Press 1992.
Zolla-Pazner, et al., "Production of Human Monoclonal Antibodies Against the V3 Loop of gp120", Sixth Int. Conference on AIDS, UCSF, San Francisco, Jun. 21, 1990--Abstract Th.A.75.
Grunow, R. et al., "The High Efficiency, Human B Cell Immortalizing Heteromyeloma CB-F7", Journal of Immunological Methods, 106(2):257-265 (Feb. 10, 1988).
Akerblom, et al., "Neutralizing Cross-Reactive and Non-Neutralizing Monoclonal Antibodies to HIV-1 gp120", AIDS 4:953-960 (1990).
Berman, P. et al., "Protection of Chimpanzees from Infection of HIV-1 After Vaccination with Recombinant Glycoprotein gp120 but not gp160", Nature 345:622-625 (1990).
Emini, E. et al., "Antibody-Mediated in Vitro Neutralization of Human Immunodeficiency Virus Type 1 Abolishes Infectivity for Champanzees" J. Virol 64(8):3674-3678 (1990).
Girard, M. et al., "Immunization of Chimpanzees Confers Protection Against Challenge with Human Immunodeficiency Virus" Proc. Natl. Acad. Sci. USA 88:542-546 (1991).
Jackson, et al., "Passive Immunoneutralisation of Human Immunodeficiency Virus in Patients with Advanced AIDS", The Lancet ii:647 (1988).
Karpas, et al. "Effects of Passive Immunization in Patients with the Acquired Immunodeficiency Syndrome-Related Complex and Acquired Immunodeficiency Syndrome" Proc. Natl. Acad. Sci. USA 85:9234-9237 (1988).
Ohlin, et al., "Human Monoclonal Antibodies Against a Recombinant HIV Envelope Antigen Produced by Primary In Vitro Immunization" Immunology 68:325-331 (1989).
Ohno, et al., "A Broadly Neutralizing Monoclonal Antibody that Recognizes the V.sub.3 Region of Human Immunodeficiency Virus Type 1 Glycoprotein gp120" Proc. Natl. Acad. Sci. USA 88:10726-10720 (1991).
Prince, et al., "Prevention of HIV Infection by Pasisve Immunization with HIV Immunoglobulin" Aids Research and Human Retroviruses 7(12):971-973 (1991).
Rusche, et al., "Antibodies that Inhibit Fusion of Human Immunodeficiency Virus-Infected Cells Bind a 24-Amino Acid Sequence of the Viral Envelope, gp120" Proc. Natl. Acad. Sci. USA 85:3198-3202 (1988).
Scott, et al., "Human Monoclonal Antibody that Recognizes the V3 Region of Human Immunodeficiency Virus gp120 and Neutralizes the Human T-Lymphotropic Virus Type III.sub.MN Strain" Proc. Natl. Acad. Sci. USA 87:8597-8601 (1990).
Berzofsky, J. et al. "Development of Artificial Vaccines Against HIV Using Defined Epitopes" The FASEB Journal 5:2414-2418 (1991).
Berzow, D. et al, "Human Monoclonal Antibodies to HIV" Proc. Vth Int'l AIDS Conf., Montreal, 1989, abstr. TCP 150.
Chanh, T. et al., "Induction of Anti-HIV Neutralizing Antibodies by Synthetic Peptides" EMBO J. 5:3065-3071 (1986).
Goudsmit, J. et al., "Human Immunodeficiency Virus Type 1 Neutralization Epitope with Conserved Architecture Elicits Early Type-Specific Antibodies in Experimentally Infected Chimpanzees" Proc. Natl. Acad. Sci. USA 85:4478-4482 (1988).
Ho, D. et al., "Conformational Epitope on gp120 Important in CD4 Binding and Human Immunodeficiency Virus Type 1 Neutralization Identified by a Human Monoclonal Antibody" J. Virol. 65(1):489-493 (1991).
Kang, C et al., "Evidence for non-V3-Specific Neutralizing Antibodies that Interfere with gp120/CD4 Binding in Human Immunodeficiency Virus 1-Infected Humans" Proc. Natl. Acad. Sci. USA 88:6171-6175 (1991).
LaRosa, G. et al., "Conserved Sequence and Structural Elements in the HIV-1 Principal Neutralizing Determinant" Science 249:932-935 (1990).
Nara, P. et al., "Emergence of Viruses Resistant to Neutralization by V3-Specific Antibodies in Experimental Human Immunodeficiency Virus Type 1 IIIB Infection of Chimpanzees" J. Virol. 64(8):3779-3791 (1991).
Putkonen, P. et al., "Prevention of HIV-2 and SIV.sub.sm Infection by Passive Immunization in Cynomolgus Monkeys" Nature 352:436-438 (1991).
Karwowska, S. et al., "Relation Between Cross-Reactivity and Affinity of Human Monoclonal Antibodies (HumAbs) to Synthetic Peptides of the V3 Loop of Divergent HIV Strains", VII International Conference on AIDS, Florence Jun. 16-21, 1991, vol. 2, Abstract #W.A.1318, p. 171.
Gorny, M.K., et al., "Human Monoclonal Antibodies (HumAbs) to the V3 Loop of HIV-1 React with Divergent Strains", VII International Conference on AIDS, Florence Jun. 16-21, 1991, vol. 2, Abstract #W.A.1319 p. 171.

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