Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1987-11-09
1989-06-27
Moskowitz, Margaret
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
4351722, 4351723, 435 68, 43524027, 530387, 436540, 436513, 436512, 436547, 935 92, 935 96, 935100, 935104, G01N 3377
Patent
active
048429988
ABSTRACT:
Human-nonhuman heterohybridomas capable of expressing IgM type antibodies can be screened to select hybridomas expressing IgM antibodies comprising human J chain components. Method comprises contacting separate samples of IgM antibodies (or IgM J chain components) from a given cell line with anti-human J chain antibodies and anti-non-human J chain antibodies to determine which antibody complexes with the J chain component of the samples, thereby identifying and permitting the early selection of a heterohybridoma expressing IgM having a human J chain component.
REFERENCES:
Kobayashi, K. et al. "Improved Procedure for the Isolation of J-chain from Human Polymeric Immunoglobulins,"Biochim. Biophys. Acta. 303:105-117, 1973.
Herbert, W. J. et al. (eds.), Dictionary of Immunology, Blackwell Scientific Publications, Oxford, 1985, pp. 110 and 148.
Kuo, M. et al. "Rabbit-Mouse Hybridomas Secreting Intact Rabbit Immunoglobulin," Molecular Immunology 22(4):351-359, 1985.
Goding, J. W., Monoclonal Antibodies:Principles and Practice, Academic Press, Inc., Orlando, Fla., 1983, pp. 175-178.
Meng Y. Gloria
Trawinski Jurgen
Cheney Kay E.
Giblin James A.
Miles Inc.
Moskowitz Margaret
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