Heterogeneous immunoassay for digoxin using ouabain as a separat

Details Heterogeneous immunoassay for digoxin using ouabain as a separat Heterogeneous immunoassay for digoxin using ouabain as a separat

1984-01-18

1985-11-05

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 14, 435810, 436512, 436536, 436541, 436542, 436804, 436808, 436815, 436823, G01N 3354, G01N 3356

Patent

active

045514267

ABSTRACT:
A heterogeneous immunoassay for digoxin. An excess of labeled anti-digoxin antibody is added to a test sample and the resulting reaction mixture is contacted with a solid phase having ouabain immobilized thereon. All the free, labeled antibody binds to the oubain. The eluted digoxin anti-digoxin antibody complex is measured for label activity. A competitive mode is also disclosed.

REFERENCES:
patent: 4039652 (1977-08-01), Adams
patent: 4064227 (1977-12-01), Brown
patent: 4200436 (1980-04-01), Mochida
patent: 4397960 (1983-08-01), Moussebois
"Methods in Enzymology", vol. 84, Immunochemical Techniques, Part D, J. J. Langone et al., eds., Chapt. 42 by V. P. Butler, Jr. et al., Immunoassay of Digoxin and Other Cardiac Glycosides, pp. 558-577, Academic Press, New York, 1982.

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