Heteroduplex tracking assay (HTA) for genotyping HCV

Details Heteroduplex tracking assay (HTA) for genotyping HCV Heteroduplex tracking assay (HTA) for genotyping HCV

1996-04-19

1998-12-22

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 6, 435 912, 435 9151, 536 2433, 935 8, 935 99, 935 98, C12Q 170, C12Q 168, C12P 1934, G07H 2104

Patent

active

058517596

ABSTRACT:
A heteroduplex tracking assay (HTA), a hybridization based method of determining the genetic relationship between two or more viral genomes, for genotyping HCV is disclosed. The HTA for genotyping HCV was developed using single stranded probes derived form the carboxyl terminus of core and part of the E1 for HCV subtypes 1a, 1b, 2a, 2b, and 3a. HTA is more accurate than RFLP for sub-typing HCV and has potential for identifying new variants and is useful for epidemiological studies.

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