Heteroduplex PCR cloning

Details Heteroduplex PCR cloning Heteroduplex PCR cloning

1999-03-10

1999-12-14

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 4353201, 536 2433, C12Q 168, C12P 1934, C12N 1500, C07H 2104

Patent

active

060016124

ABSTRACT:
The invention relates to a method of cloning a target nucleic acid sequence by performing two PCR reactions to generate two PCR products differing in terminal sequences. The PCR products thus obtained are denatured and annealed together to form a heteroduplex, which is ready for ligation and transformation of bacteria without any restriction digestion.

REFERENCES:
Liu, "Hetero-stagger Cloning: Efficient and Rapid Cloning of PCR Products", Nucleic Acids Research 24:2458-2459, 1996.
Holton et al., "A Simple and Efficient Method for Direct Cloning of PCR Products Using ddT-tailed Vectors", Nucleic Acids Research 19:1156, 1990.
Marchuk et al., "Construction of T-vectors, a Rapid and General System for Direct Cloning of Unmodified PCR Products", Nucleic Acids Research 19:1154, 1990.
Shuldiner et al., "PCR-induced (Ligase-free) Subcloning: A Rapid Reliable Method to Subclone Polymerase Chain Reaction (PCR) Products", Nucleic Acids Research 18:1920, 1990.

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