Heterodimers of retinoid X receptors (RXRS) and other...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007200, C435S069100, C435S252300, C435S320100, C435S325000, C435S471000

Reexamination Certificate

active

07026125

ABSTRACT:
This invention provides a purified heterodimer comprising an RXR and a hormone receptor. The invention also provides a method of screening ligands for their effect on the activity of an RXR-containing hormone receptor heterodimer comprising combining the heterodimer with the ligand and determining the effect on activity. Also provided is a method of amplifying the activity of a hormone receptor comprising forming a heterodimer with another hormone receptor.

REFERENCES:
patent: 5654137 (1997-08-01), Astrom et al.
patent: WO 91/12258 (1991-08-01), None
patent: WO 93/11235 (1993-06-01), None
Mangelsdorf DJ, et al. Nature 345:224-228, 1990.
Glass CK, et al. Cell 59:697-708, 1989.
Darling et al., “3,5,3′-Triiodothyronine (T3) receptor-auxiliary protein (TRAP) binds DNA and forms heterodimers with the T3receptor,”Mol. Endo.5(1):73-84 (1991).
Glass et al., “Multiple cell type-specific proteins differentially regulate target sequence recognition by the α retinoic acid receptor,”Cell63:729-738 (1990).
Glass et al., “Positive and negative regulation of gene transcription by a retinoic acid-thyroid hormone receptor heterodimer,”Cell59:697-708 (1989).
Hamada et al., “H-2RIIBP, a member of the nuclear hormone receptor superfamily that binds to both the regulatory element of major histocompatibility class I genes and the estrogen response element,”Proc. Natl. Acad. Sci.(USA) 86:8289-8293 (1989).
Mangelsdorf et al., “Nuclear receptor that identifies a novel retinoic acid response pathway,”Nature345:224-229 (1990).
Yu et al., “RXRβ: a coregulator that enhances binding of retinoic acid, thyroid hormone, and vitamin D receptors to their cognate response elements,”Cell67:1251-1266 (1991).

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