Heterodimeric osteogenic factor

Details Heterodimeric osteogenic factor Heterodimeric osteogenic factor

1993-11-08

1995-05-02

Hill, Jr., Robert J.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

514 21, 530399, 530350, C07K 1300

Patent

active

054119413

ABSTRACT:
The present invention provides osteogenically active protein preparations comprising a heterodimer of P3 OF 31-34 subunit B and P3 OF 31-34 subunit D, which subunits are linked with at least one disulfide bond and methods for their preparation. The invention further provides cell lines transformed with nucleotide sequences encoding P3 OF 31-34 subunit B and P3 OF 31-34 subunit D and vectors comprising those sequences in operative association with an expression control sequence.

REFERENCES:
patent: 3458397 (1969-07-01), Myers et al.
patent: 4294753 (1981-10-01), Urist
patent: 4314380 (1982-02-01), Miyata et al.
patent: 4394370 (1983-07-01), Jefferies
patent: 4407787 (1983-10-01), Stemberger
patent: 4430760 (1984-02-01), Smestad
patent: 4434094 (1984-02-01), Seyedin et al.
patent: 4440750 (1984-04-01), Glowacki et al.
patent: 4455256 (1984-06-01), Urist
patent: 4488911 (1984-12-01), Luck et al.
patent: 4500707 (1985-02-01), Caruthers et al.
patent: 4563350 (1986-01-01), Nathan et al.
patent: 4563489 (1986-01-01), Urist
patent: 4608199 (1986-08-01), Caplan et al.
patent: 4619989 (1986-10-01), Urist
patent: 4627982 (1986-12-01), Seyedin et al.
patent: 4681763 (1987-07-01), Nathanson et al.
patent: 4725536 (1988-02-01), Fritsch et al.
patent: 4734363 (1988-03-01), Dattagupta et al.
patent: 4757006 (1988-07-01), Toole, Jr. et al.
patent: 4761471 (1988-08-01), Urist
patent: 4774228 (1988-09-01), Seyedin et al.
patent: 4774322 (1988-09-01), Seyedin et al.
patent: 4789732 (1988-12-01), Urist
patent: 4804744 (1989-02-01), Sen
patent: 4863899 (1989-09-01), Todaro
patent: 4877864 (1989-10-01), Wang et al.
patent: 4952404 (1990-08-01), Valle et al.
patent: 4968590 (1990-11-01), Kuberasampath et al.
patent: 4975526 (1990-12-01), Kuberasampath et al.
patent: 5001169 (1991-03-01), Nathan et al.
patent: 5002770 (1991-03-01), Kuberasampath et al.
patent: 5011691 (1991-04-01), Opperman et al.
patent: 5013649 (1991-05-01), Wang et al.
patent: 5017486 (1991-05-01), Sawai et al.
patent: 5106748 (1992-04-01), Wozney et al.
patent: 5108922 (1992-04-01), Wang et al.
patent: 5116738 (1992-05-01), Wang et al.
patent: 5141905 (1992-08-01), Rosen et al.
patent: 5166058 (1992-11-01), Wang et al.
patent: 5168050 (1992-12-01), Hammonds, Jr. et al.
patent: 5182365 (1993-01-01), Opperman et al.
patent: 5187076 (1993-02-01), Wozney et al.
Amitani et al., "Studies on a Factor Responsible for New Bone Formation from Osteosarcoma in Mice," Calcif. Tiss. Res., 17, 139-150 (1975).
Assoian et al, "Transforming Growth Factor-.beta. in Human Platelets," J. Biol. Chem., 258, 7155-7560 (1983).
Balland et al., "Use of synthetic oligonucleotides in gene isolation and manipulation," Biochimie, 67, 725-736 (1985).
Bauer et al., "Human Osteosarcoma-Derived Soluble Bone Morphogenetic Protein," Clin. Ortho. Rel. Res., 154, 291-295 (Jan., Feb. 1981).
Baylink et al, "The Regulation of Endosteal Bone Volume," J. Periodontal., 1, 43-49 (1979).
Bentz et al., "Purification and Characterization of a Unique Osteoinductive Factor From Bovine Bone," J. Bone and Mineral Res., 4, Supplement 1, p. S280, No. 650(1989).
Bentz et al., "Purification of an Osteoinductive Factor From Bovine Demineralized Bone," J. Cell. Biol., 107, 162a, No. 918 (1989).
Bentz et al., "Amino Acid Sequence of Bovine Osteoinductive Factor," J. Biol. Chem., 265, pp. 5024-5029 (1990).
"Australian institutes join to develop bio-products," Biotechnology Newswatch, 9, No. 23, 1, 3 (Dec. 4, 1989).
Bitter et al., "Expression of heterologous genes in Saccharomyces cerevisiae from vectors utilizing the glyceralde-hyde-3-phosphate dehydrogenase gene promoter," Gene, 32, 263-274 (1984).
Celeste et al., "Identification of transforming growth factor .beta. family members present in bone-inductive protein purified from bovine bone," Proc. Natl. Acad. Sci. USA, 87, pp. 9843-9847 (Dec., 1990).
Cepko et al., "Construction and applications of a Highly Transmissible Murine Retrovirus Shutte Vector," Cell, 37, 1053-1062 (Jul., 1984).
Conover et al., "Dentin Matrix Bone Morphogenetic Protein," The Chemistry and Biology of Mineralized Connective Tissues, 597-606, Elsevier (1981).
Drivdahl et al., "Regulation of DNA Synthesis in Chick Calvaria Cells by Factors from Bone Organ Culture," Proc. Soc. Exptl. Biol. Med., 168, 143-150 (1981).
Edge, "Total synthesis of a human leukocyte interferon gene," Nature, 292, 756-762 (Aug., 1981).
Farley et al., "Purification of a Skeletal Growth Factor from Human Bone," Biochemistry, 21, No. 14, 3502-3507 (1982).
Farley et al., "Human Skeletal Growth Factor: Characterization of the Mitogenic Effect on Bone Cells in Vitro," Biochemistry, 21. No. 14, 3508-3513 (1982).
Gorman et al., "Recombinant Genomes Which Express Chloramphenicol Acetyltransferase in Mammalian Cells," Mol. Cell. Biol., 2, 1044-1051 (Sep., 1982).
Hanamura et al. "Solubilized Bone Morphogenetic Protein (BMP) from Mouse Osteosarcoma and Rat Demineralized Bone Matrix," Clin. Ortho. Res., 148, 281-290 (1980).
Hanamura et al. "Solubilization and Purification of Bone Morphogenetic Protein (BMP) from Dunn Osteosarcoma," Clin. Ortho. & Rel. Res., 153, 232-240 (1980).
Harakas, "Demineralized Bone-Matrix-Induced Osteogenesis," Clin. Ortho. Rel. & Res, 188, 239-251 (1984).
Hauschka et al., "Direct identification of the calcium-binding amino acid, .gamma.-carboxyglutamate, in mineralized tissue," Proc. Natl. Acad. Sci., 72, 3925-3929 (Oct., 1975).
Howard et al., "Evidence for the Coupling of Bone Formation to Bone Resorption In Vitro," Metabolic Bone Desease Rel. Res., 2, 131-135 (Oct., 1980).
Israel et al., "Expression of Recombinant BMP2 in Chinese Hamspter Ovary Cells," J. Cell. Biochem., Suppl. 15F, p. 168, Abstract No. Q11 (1991).
Kubersampath et al., "Bovine Osteogenic Protein Is Composed of Dimers of OP-1 and BMP-2A, Two Members of the Transforming Growth Factor-.beta.Superfamily," J. Biol. Chem., 265, pp. 13198-13205 (1990).
Luyten et al., "Purification and Partial Amino Acid Sequence of Osteogenin, a Protein Initiating Bone Defferentiation," J. Biol. Chem., 264, 13377-13380 (1989).
Lyons et al., "Vgr-1, a mammalian gene related to Xenopus Vg-1, is a member of the transforming growth factor .beta. gene superfamily," Proc. Natl. Acad. Sci., 86, 4554-4558 (Jun., 1989).
Maugh, "Human Skeletal Growth Factor Isolated," Science, 217, 819 (Aug., 1982).
Mbuyi et al., "Plasma Proteins in Human Cortical Bone: Enrichment of .alpha..sub.2 HS-Glyprotein, .alpha..sub.1 Acid-Glycoprotein, and IgE," Calcif. Tiss. Int., 34, 229-231 (1982).
Mizutani and Urist, "The Nature of Bone Morphogenetic Protein (BMP) Fractions Derived from Bovine Bone Matrix Gelatin," Clin. Ortho. & Rel Res., 171, 213-223 (Nov., Dec., 1982).
Nogami et al., "Radioactive Isotope Labeled Diffusible Components of a Bone Morphogenetic Substratum," Clin. Ortho. Rel. Res., 122, 307-314 (Jan., Feb., 1977).
Ozkaynak et al., "OP-1 cDNA encodes an osteogenic protein in the TGF-.beta. family," The EMBO Journal, 9, pp. 2085-2093 (1990).
Price et al., "Characterization of a .gamma.-carboxyglutamic acid-containing protein from bone," Proc. Natl. Acad. Sci., 73, 1447-1451 (May, 1976).
Raisz et al., "Regulation of Bone Formation, (First of Two Parts)," New England J. Med., 309, No. 1, 29-35 (Jul. 7, 1983).
Raisz et al., "Regulation of Bone Formation, (Second of Two Parts)," New England J. Med., 309, No. 2, 83-89 (Jul. 14, 1983).
Reddi, "Cell Biology and Biochemistry of Endochondral Bone Development," Coll. Res., 1, 209-226 (1981).
Sampath et al., "Homology of bone-inductive proteins from human, monkey, bovine, and rat extracelluar matrix," Proc. Natl. Acad. Sci., 80, 6591-6595 (Nov. 1983).
Sen et al., "Purification and Partial Characterization of a Novel Osteogenic Protein," in Development and Diseases of Cartilage and Bone Matrix, pp. 201-220 (1987).
Seyedin et al., "Purification and characterization of two cartilage-inducing factors from bovine demineralized bone," Proc. Natl. Acad. Acad. Sci., 82, 2267-2271 (Apr., 1985).
Takaoka et al., "Solubilization and Concentration of a Bone-Inducing Substance from a Murine Osteosarcoma," Clin. Ortho. & Rel. R

Affiliated with

Also associated with

Rating

Heterodimeric osteogenic factor does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Heterodimeric osteogenic factor, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Heterodimeric osteogenic factor will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1137064