Heterobifunctional crosslinked agents for immobilizing molecules

Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...

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435 792, 436532, 530816, 560190, C12N 1106, G01N 33549, C07C 6934

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active

054361478

ABSTRACT:
Heterobifunctional crosslinking agents are synthesized that covalently link molecules such as enzymes, cells, proteins and nucleic acids to a plastic substrate. The agents contain a central ring structure having a hydrophobic hydrocarbon chain that binds to a plastic substrate and distal to the hydrophobic chain one or more hydrophilic chains terminating in a reactive group that covalently binds the molecule. Immobilized molecules are useful in diagnostic assays or bioreactors. Preferred heterobifunctional crosslinking agents have the following structures: ##STR1##

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patent: 4808530 (1989-02-01), Means et al.
patent: 4889916 (1989-12-01), Packard et al.
patent: 5002883 (1991-03-01), Bieniarez et al.

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