Hetero type pentamer recombinant vaccine

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Conjugate or complex

Reexamination Certificate

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C424S186100, C424S192100, C424S193100, C424S196110, C424S201100, C530S350000, C536S023100, C435S320100, C435S252300, C435S069300, C435S069700

Reexamination Certificate

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07544361

ABSTRACT:
A heteropentamer composed of a fusion monomer (32) comprising a fusion protein of an antigenic amino acid sequence and an amino acid sequence of a monomer of a mucous membrane-binding protein and a nonfusion monomer (20) of an amino acid sequence of a monomer of a mucous membrane-binding protein. A homopentamer composed of a fusion monomer including a fusion protein of an antigen derived from an envelope protein of Japanese encephalitis virus and an amino acid sequence of a monomer of a mucous membrane-binding protein. These heteropentamer and the homopentamer can be used as a vaccine.

REFERENCES:
patent: 5589384 (1996-12-01), Lipscombe et al.
patent: 2008/0286297 (2008-11-01), Florack et al.
patent: 3-178995 (1991-08-01), None
patent: 6-206900 (1994-07-01), None
patent: 2003-116385 (2003-04-01), None
Jobling et al (Infection and Immunity 70:1260-1271, 2002).
Wu et al (Journal of Biological Chemistry 278: 46007-13, 2003).
Sissela Liljeqvist, et al. “Fusions to the cholera toxin B subunit: influence on pentamerization and GM1 binding” Journal of Immunological Methods, vol. 210, No. 2, XP-004112016, Dec. 29, 1997, pp. 125-135.
Tetsuya Harakuni, et al. “Heteropentameric Cholera Toxin B Subunit Chimeric Molecules Genetically Fused to a Vaccine Antigen Induce Systemic and Mucosal Immune Responses: a Potential new Strategy To Target Recombinant Vaccine Antigens to Mucosal Immune Systems” infection and Immunity vol. 73, No. 9, XP-002461843, Sep. 2005, pp. 5654-5665.

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