Chemistry: molecular biology and microbiology – Vector – per se
Patent
1993-01-21
1994-06-28
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Vector, per se
435 691, 4352351, 4351721, 4351723, 536 231, 536 2372, 536 241, 530350, 424 93A, C12N 1586, C12N 1538, A61K 4500
Patent
active
053246640
ABSTRACT:
Methods for isolating thymidine kinase-encoding DNA of a herpes virus are described. These methods utilize degenerate primers based on regions of relatively conserved amino acid sequence in herpes virus thymidine kinase proteins to initiate a polymerase chain reaction which yields large amounts of the thymidine kinase-encoding DNA. The methods are illustrated in the isolation of the thymidine kinase gene of feline herpes virus, which can be used to construct recombinant thymidine kinase-negative feline herpes viruses for purposes of constructing live vaccines and expression vectors. In addition, the regulatory elements of the feline herpes virus thymidine kinase gene are useful in the construction of recombinant DNA vectors.
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Compton Teresa
Nunberg Jack H.
Petrovskis Erik A.
Post Leonard E.
Darnely, Jr. James D.
Guzo David
Schwartz Richard A.
Steele Gregory W.
The Upjohn Company
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