Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1994-11-21
1997-08-19
Guzo, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
4351723, 4352351, 435325, 4353201, 435236, 435364, C12Q 170, C12N 704, C12N 510, C12N 1586
Patent
active
056587244
ABSTRACT:
Novel cell lines that express complementing levels of herpes simplex virus (HSV) essential immediate early proteins ICP4 and ICP27 and a method of producing the novel cell lines. These novel cell lines are utilized to provide novel HSV strains deficient for both ICP4 and ICP27, and their generation, and novel HSV strains deficient for ICP4 and ICP27 and one or more additional genes, and their generation. Vectors are provided from these methods of using these vectors for gene transfer and for producing site-specific homologous recombinations with cellular DNA.
REFERENCES:
patent: 5026635 (1991-06-01), Ferguson et al.
patent: 5070010 (1991-12-01), Hsu
patent: 5124263 (1992-06-01), Temin et al.
Johnson et al., "Improved Cell Survival by the Reduction of Immediate-Early ene Expression in Replication-Defective Mutants of Herpes Simplex Virus Type 1 but Not by Mutation of the Virus Host Shutoff Function", J. Virol., Oct. 1994, pp. 6347-6362.
Werstuck et al., J. Of Virol., Mar. 1990, pp. 984-991, vol. 64, No. 3.
Kmetz et al., Nucleic Acid Res., vol. 16, No. 10, 1988, p. 4735.
Shih et al., PNAS, vol. 81, Sep. 1984, pp. 5867-5870.
Brakefield, X.O. and DeLuca, N.A., 1991, Herpes Simplex as a Vector for Neurons, in: Treatment of Genetic Diseases, Ed: Desnick, R.J., pp. 287-319; Churchill Livingston.
Chiocca, E. A., et. al., 1990, Transfer and Expression of the LacZ gene in Rat Brain Neurons Mediated by Herpes Simplex Virus Mutants. The new Biologist 2(8):739-746.
Breakfield, X.O. and DeLuca, N.A., 1991, Herpes Simplex Virus for Gene Delivery to Neurons, The New Biologist 3(3): 203-218.
DeLuca, N. A., et al., 1985, Isolation and Characterization of Deletion Mutants of HSV-1 in the Gene Encoding Immediate-Early Regulatory Protein ICP24. J. Virol. 56:558-570.
DeLuca, N.A. and Schaffer, P.A., 1987, Activities of Herpes Simplex Virus type 1 (HSV-1) ICP24 Genes Specifying Nonsense Peptides. Nucleic Acids Res. 15(11):4491-4511.
DeLuca, N.A. and Schaffer, P.A., 1988, Physical and Functional Domains of the Herpes Simplex Virus Transcriptional Regulatory Protein ICP4. J. Virol. 62:732-743.
Imbalzano, A.N., et al., 1991, Herpes Simplex Virus Transactivator ICP4 Operationally Substitues for the Cellular Transcription Factor SP1 for Efficient Expression of the Viral Thymidine Kinase Genes. J. Virol. 65:565-574.
McCarthy, A.M., et al., 1989, Herpes Simplex Virus Type 1 ICP27 Deletion Mutants Exhibit Altered Patterns of Transcription and are DNA Deficient. J. Virol. 63:18-27.
Paterson, T. and Everett, R.D., 1988, Mutational Dissection of the HSV-1 Immediate-early Protein Vmw175 Involved in Transcriptional Transactivation and Repression. Virology 166:186-186.
Paterson, T. and Everett, R.D., 1988, The Regions of the Herpex Simplex Virus Type 1 Immediate-Early Protein Vmw175 Required for Site Specific DNA Binding Closely Correspond to Those Involved in Transcriptional Regulation. Nucleic Acids Res. 16(23):11005-11025.
Shepard,A.A. et al., 1989, Separation of Primary Structural Components Conferring Autoregulation, Transactivation, and DNA-Binding Properties to the Herpes Simplex Virus Transcriptional Regulatory Protein ICP4. J. Virol. 63:3714-3728.
Shepard,A.A., et al., 1991, A Second-Site Revertant of a Defective Herpes Simplex Virus ICP4 Protein with Restored Regulatory Activities and Impaired DNA-Binding Properties. J. Virol. 65:787-795.
Guzo David
University of Pittsburgh of the Commonwealth System of Higher Ed
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