Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1996-07-26
1999-03-02
Stucker, Jeffrey
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 6, 435 692, 424 931, 424 932, 514 44, C12Q 170, C12Q 168, A01N 6300, A01N 4304
Patent
active
058769234
ABSTRACT:
The ICP4 protein of herpes simplex virus plays an important role in the transactivation of viral genes. The present invention discloses that ICP4 also has the ability to inhibit apoptosis. This function appears to reside in functional domain distinct from the transactivating function, as indicated by studies using temperature sensitive mutants of ICP4 that transactivating function at elevated temperatures. Also disclosed are methods for inhibition of apoptosis using ICP4 or an ICP4 encoding gene, such as an .alpha.4 gene, methods of inhibiting ICP4's apoptosis-inhibiting function, and methods for the production of recombinant proteins and treatment of HSV infections.
REFERENCES:
patent: 5500432 (1996-03-01), Nicolaou et al.
International Search Report dated Feb. 3, 1998 (PCT/US97/12904) (ARCD:239P).
Leopardi and Roizman, "Functional interaction and colocalization of the herpes simplex virus 1 major regulatory protein ICP4 with EAP, a nucleolar-ribosomal protein," Proc. Natl. Acad. Sci. USA, 93:4572-4576, 1996.
Leopardi and Roizman, "The herpes simplex virus major regulatory protein ICP4 blocks apoptosis induced by the virus or by the hyperthermia," Proc. Natl. Acad. Sci. USA, 93:9583-9587, 1996.
Papavassiliou, Wilcox and Silverstein, "The interaction of ICP4 with cell/infected-cell factors and its state of phosphorylation modulate differential recognition of leader sequences in herpes simplex virus DNA," EMBO Journal, 10(2):397-406, 1991.
Xia, Knipe and DeLuca, "Role of protein kinase A and the serine-rich region of herpes simplex virus type 1 ICP4 in viral replication," Journal of Virology, 70(2):1050-1060, 1996.
Baichwal and Sugden, "Vectors for Gene Transfer Derived from Animal DNA Viruses: Transient and Stable Expression of Transferred Genes,"In: Gene Transfer, Kucherlapti (Ed.), New York: Plenum Press, 1986.
Batterson and Roizman, "Characterizationof the Herpes Simplex Virion-Associated Factor Responsible for the Induction of .alpha. Genes,"J Virology, 46(2):371-377, May 1983.
Benevenisty and Reshef, "Direct Introduction of Genes into Rats and Expressionof the Genes," Proc. Natl. Acad. Sci., USA, 83:9551-9555, Dec. 1986.
Bitter et al., "Expression and Secretion Vectors for Yeast," Methods in Enzym, 153:516-444, 1987.
Dubensky et al., "Direct Transfection of Viral and Plasmid DNA into the Liver of Spleen of Mice," Proc. Natl. Acad. Sci., USA, 81:7529-7533, Dec. 1984.
Ejercito et al., "Characterization of Herpes Simplex Virus Strains Differing in their Effects on Social Behaviour of Infected Cells," J Gen Virol, 2:357-364, 1968.
Faber and Wilcox, "Asociation of the Herpes Simplex Virus Regulatory Protein ICP4 with Specific Nucletide Sequences in DNA," Nucleic Acids Research, 14:6067-6083, Nov. 1986.
Fechheimer et al., Transfection of Mammalian Cells with Plasmid DNA by Scrape Loading and Sonication Loading, Proc. Natl. Acad. Sci., USA, 84:8463-8467, Dec. 1987.
Ferkol et al., "Regulation of the Phosphoenolpyruvate Carboxykinase/Human Factor IX Gene Introduced Into the Livers of Adult Ratsz by Receptor-Mediated Gene Transfer," FASEB Journal, 7:1081-1091, Aug. 1993.
Fetrow and Bryant, "New Programs for Protein Tertiary Structure," Bio/Technology, 11:479-484, Apr. 1993.
Fraley et al., "Entrapment of a Bacterial Plasmid in Phospholipid Vesicles: Potential for Gene Transfer," Proc. Natl. Acad. Sci., USA, 76(7):3348-3352, 1979.
Ghosh and Bachhawat, "Targeting of Liposomes to Hepatocytes," In: Liver Diseases Targeted Diagnosis and Therapy Using Specific Receptors and Ligands, Wu and Wu (Eds.), New York: Marcel Dekker, Inc., 1991.
Glorioso et al., "Development and Application of Herpes Simplex Virus Vectors for Human Gene Therapy," Annu Rev Mircobiol, 49:675-710, 1995.
Gopal, "Gene Transfer Method for Transient GeneExpression, Stable Transformation, and Cotransformation of Suspension Cell Cultures," Molecular and Cellular Biology, 5(5):1188-1190, May 1985.
Gorczyca et al., "Detection of DNA Strand Breaks in Individual Apoptotic Cells by the in Situ Terminal Deoxynucleotodyl Transferase and Nick Translation Assays," Cancer Research, 53:1945-1951, Apr. 1993.
Brutlag et al., "Improved Sensitivity of Biological Sequence Database Searches," Cabios, 6(3):237-245, 1990.
Campbell et al., "Identification of Herpes Simplex Virus DNA Sequences which Encode a Trans-acting Polypeptide Responsible for Stimulation of Immediate Early Transcription," J Mol Biol, 180:1-19, 1984.
Chen and Okayama, "High-Efficiency Transformation of Mammalian Cells by Plasmid DNA," Molecular and Cellular Biology, 7(8):2745-2752, Aug. 1987.
Chou and Fasman, "Conformational Parameters for Amino Acids in Helical, .beta.-Sheet, and Random Coil Regions Calculated from Proteins," Biochem, 13(2):211-222, 1974.
Chou and Fasman, "Empirical Predictions of Protein Conformation," Ann Rev Biochem, 47:251-276, 1978.
Chou and Fasman, "Prediction of .beta.-Turns," Biophys J, 26:367-383, Jun. 1979.
Chou and Fasman, "Prediction of Protein Conformation," Biochem, 13(2):222-245, 1974.
Chou and Fasman, "Prediction of the Secondary Structure of Proteins from their Amino Acid Sequence," In: Advances in Enzymology, Meister (Ed..), New York: John Wiley & Sons, vol. 47:45-148, 1978.
Chou and Roizman, "The .gamma..sub.1 34.5 Gene of Herpes Simplex Virus 1 Precludes Neuroblastoma Cells from Triggering Total Shutoff of Protein Synthesis Characteristic of Programmed Cell Death in Neuronal Cells," Proc. Natl. Acad. Sci., USA, 89:3277-3270, Apr. 1992.
Conley et al., "Molecular Genetics of Herpes Simplex Virus," J Virol, 37(1):191-206, Jan. 1981.
DeLuca et al., "Isolation and Characterization of Deletion Mutants of Herpes Simplex Virus Type 1 in the Gene Encoding Immediate-Early Regulatory Protein ICP4," J Virology, 56(2):558-570, Nov. 1985.
Dixon and Schaffer, "Fine-Structure Mapping and Functional Analysis of Temperature-Sensitive Mutants in the Gene Encoding the Herpes Simplex Virus Type 1 Immediate Early Protein VP175," J Virology, 36(1):189-203, Oct. 1980.
Graham and Van Der Eb, "A New Technique for the Assay of Infectivity of Human Adenovirus 5 DNA,"Virology, 52:456-467, 1973.
Harland and Weintraub, "Translation of mRNA Injected into Xenopus Oocytes is Specifically Inhibited by Antisense RNA," J Cell Biol, 101:1094-1099, Sep. 1985.
Henderson et al., "Epstein-Barr Virus-Coded BHRF1 Protein, A Viral Homologue of Bcl-2, Protects Human B Cells from Programmed Cell Death," Proc. Natl. Acad. Sci., USA, 90:8479-8483, Sep. 1993.
Herz and Roizman, "The .alpha. Promoter Regulator-Ovalbumin Chimeric Gene Resident in Human Cells is Regulated Like the Authentic .alpha. 4 Gene After Infection with Herpes Simplex Virus 1 Mutants in .alpha. 4 Gene," Cell, 33:145-151, May 1983.
Holland et al., "Viral DNA synthesis is Required for the Efficient Expression of Specific Herpes Simplex Virus Type 1 mRNA Species," Virology, 101:10-24, 1980.
Honess and Roizman, "Proteins Specified by Herpes Simplex Virus XIII. Glycosylation of Viral Polypeptides," J Virol, 16(5):1308-1326, Nov. 1975.
Honess and Roizman, "Regulation of Herpesvirus Macromolecular Synthesis I. Cascade Regulation of the Synthesis of Three Groups of Viral Proteins," J Virol, 14(1):8-19, Jul. 1974.
Jameson and Wolf, "The Antigenic Index: A Novel Algorithm for Predicting Antigenic Determinants," Cabios, 4(1):181-186, 1988.
Johnson and Kahn, "Peptide Turn Mimetics," In: Biotechnology and Pharmacy, New York: Chapman and Hall, Chapter 14:366-378, 1993.
Kaneda et al., "Increased Expression of DNA Conintroduced with Nuclear Protein in Adult Rat Liver," Science, 243:375-378, Jan. 1989.
Kato et al., "Expressio of Hepatitis B Virus Surface Antigen in Adult Rat Liver," J Biol Chem, 266(6):3361-3364, Feb. 1991.
Klein et al., "High-Velocity Microprojectiles for Delivering Nucleic Acids into Living Cells," Nature, 327:70-73, May 1987.
Kristie and Roizman, ".alpha.4, The Major Regulatory Protein of Herpes Simplex Virus Type 1, is Stably and Specifically Associated with Promoter-Regulatory Domains of .alpha. Genes and of Selected Other Viral Genes," Proc. Natl. Acad. Sci., USA, 83:3218-3222, May 1986.
Kristie and Roizman,
Leopardi Rosario
Roizman Bernard
ARCH Development Corporation
Park Hankyel T.
Stucker Jeffrey
LandOfFree
Herpes simplex virus ICP4 as an inhibitor of apoptosis does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Herpes simplex virus ICP4 as an inhibitor of apoptosis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Herpes simplex virus ICP4 as an inhibitor of apoptosis will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-421280