Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-07-07
1997-08-05
Mosher, Mary E.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435348, 4352523, 4353201, 4352351, 435365, 530350, 536 2372, 930224, 4241861, C12P 2102, C07K 14035, C12N 1538, C12N 510
Patent
active
056541740
ABSTRACT:
The present invention provides variant HSV-1 glycoprotein D and HSV-2 glycoprotein D molecules capable of preventing infection of cells by herpes simplex virus types 1 and/or 2. Also provided are novel purified and isolated polynucleotides encoding the variant gD molecules. HSV gD-1 and gD-2 region IV variants or fragments thereof are specifically contemplated by the invention. The presently preferred variant molecule gD-1(.DELTA.290-299t) is the product of recombinant expression in Sf9 cells of a fusion protein including the signal peptide of honeybee melittin and Patton strain HSV-1 gD wherein (1) the Patton strain amino acid residues 290 through 299 of the mature gD-1 protein have been replaced with the amino acid residues arginine, lysine, isoleucine and phenylalanine, and (2) Patton strain amino acid residues 308 through 369 have been replaced with five histidine residues. When expressed in Sf9 cells, cleavage of the melittin signal peptide results in the presence of aspartate and proline residues at the amino terminus of the variant molecule. The amino acid sequence of gD-1(.DELTA.290-299t) is set out in SEQ ID NO: 2 and the preferred DNA sequence encoding gD-1(.DELTA.290-299t) is set out in SEQ ID NO: 1. Administration of gD variant molecules of the invention to mammalian subjects, especially humans, for the purpose of preventing HSV infection and/or ameliorating pathological sequelae of HSV infection is specifically contemplated.
REFERENCES:
patent: 4709011 (1987-11-01), Cohen et al.
patent: 4762708 (1988-08-01), Cohen et al.
patent: 5149660 (1992-09-01), Cohen et al.
Johnson, et al. J. Virol. 62:4506-4612 1988.
Cai et al., "Herpes Simplex Virus Type 1 ICPO Plays a Critical Role in the De Novo Synthesis of Infectious Virus following Transfection of Viral DNA," J. Virol., 63(11):4579-4589 (1989).
Chiang et al. "Identification of Functional Regions of Herpes Simplex Virus Glycoprotein gD by Using Linker-Insertion Mutagenesis," J. Virol., 68(4):2529-2543 (1994).
Cohen et al. "Expression of Herpes Simplex Virus Type 1 Glycoprotein D Deletion Mutants in Mammalian Cells," J. Virol., 62(8):1932-1940 (1988).
Fields and Knipe, Eds., Fundamental Virology, Chapter 16, Raven Press, New York (1986).
Hill et al., "Trauma to the Skin Causes Recurrence of Herpes Simplex in the Mouse," J. gen. Virol., 39: 21-28 (1978).
Hill et al., "Adrenergically induced recurrent HSV-1 corneal epithelial lesions," Curr. Eye Res., 6(8): 1065-1071 (1987).
Johnson et al., "Soluble Forms of Herpes Simplex Virus Glyoprotein D Bind to a Limited Number of Cell Surgace Receptors and Inhibit Virus Entry into Cells," J. Virol., 64(6): 2569-2576 (Jun. 1990).
Kunkel et al., "Rapid and Efficient Site-Specific Mutagenesis without Phenotypic Selection," Methods Enzymol., 154: 367-382 (1987).
Landolfi et al., "Baculovirus-expressed herpes simplex virus type 2 glycoprotein D is immunogenic and protective against lethal HSV challenge," Vaccine, 11: 407-414 (1993).
Ligas and Johnson, "A Herpes Simplex Virus Mutant in Which Glycoprotein D Sequences Are Replaced by .beta.-Galactosidase Sequences Binds to but Is Unable To Penetrate into Cells," J. Virol., 62(5): 1486-1494 (1988).
Long et al., "Glycoprotein D Protects Mice Against Lethal Challenge with Herpes Simplex Virus Types 1 and 2," Infect. Immun. 37(2): 761-764 (1984).
Martin et al., "Soluble Glycoprotein D Blocks Herpes Simplex Virus Type 1 Infection of Rat Eyes," J. Virol., 66(9): 5183-5189 (Sep. 1992).
Metcalf et al., "Herpetic Keratitis in Athymic (Nude) Mice," Infect, Immun., 26(3): 1164-1171 (1979).
Muggeridge et al., "Identification of a Site on Herpes Simplex Virus Type 1 Glycoprotein D That Is Essential for Infectivity," J. Virol., 64(8): 3617-3626 (1990).
Rock and Fraser, "Detection of HSV-1 genome in central nervous system of latently infected mice," Nature, 302(7): 523-525 (1983).
Sisk et al., "High-Level Expression and Purification of Secreted Forms of Herpes Simplex Virus Type 1 Glycoprotein gD Synthesized by Baculovirus-Infected Insect Cells," J. Virol., 68(2): 766-775 (1994).
Stanberry et al., "Genital Herpes in Guinea Pigs: Pathogenesis of the Primary Infection and Description of Recurrent Disease," J. Infect. Dis., 146(3): 397-404 (Sep. 1982).
Stanberry, L.R., "Pathogenesis of Herpes Simplex Virus Infection and Animal Models for its Study," Current Topics in Microbiol. and Immuno., 179: 15-30 (1992).
Stevens, "Humans Herpesviruses: a Consideration of the Latent State," Microbiol. Rev., 53(3): 318-332 (1989).
Stevens and Cook, "Latent Herpes Simplex Virus in Spinal Ganglia of Mice," Science, 173: 843-845 (1971).
Straus et al., "Suppression of Frequently Recurring Genital Herpes, A Placebo-Controlled Double-Blind Trial of Oral Acyclovir," N. Eng. J. Med., 310(24): 1545-1550 (1984).
Tal-Singer et al., "Interaction of Herpes Simplex Virus Glycoprotein gC with Mammalian Cell Surface Molecules" J. Virol., 69(7): 4471-4483 (1995).
Tenser, R.B., "Intracerebral Inoculation of Newborn and Adult Mice with Thymidine Kinase-Deficient Mutants of Herpes Simplex Virus Type 1," J. Infect. Dis., 147: 956 (May 1983).
Tessier et al., "Enhanced secretion from insect cells of a foreign protein fused to the honeybee melittin signal peptide," Gene, 98: 177-183 (1991).
van Regenmortel and Neurath Eds., Chapter 20 in Immunochemistry of Viruses, II. The Basis for Serodiagnosis and Vaccines, Elsevier Science Publishers B.V. (1990).
Watson et al., "Herpes Simplex Virus Type-1 Glycoprotein D Gene: Nucleotide Sequence and Expression in Escherichia coli," Science, 218(22): 381-384 (Oct. 1982).
Cohen Gary H.
Eisenberg Roselyn J.
Nicola Anthony
Chen Michael
Competitive Technologies Inc.
Mosher Mary E.
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