Herbicide-tolerant plants expressing carbamate hydrolase

Details Herbicide-tolerant plants expressing carbamate hydrolase Herbicide-tolerant plants expressing carbamate hydrolase

1990-11-19

1994-09-13

Chereskin, Che S.

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide alters fat, fatty oil, ester-type wax, or...

800DIG43, 4351723, 4353201, 935 64, 935 67, A01H 400, C12N 1582

Patent

active

053470761

ABSTRACT:
A process for the isolation and characterization of a gene enzyme system for the inactivation of the herbicide phenmedipham, wherein the enzyme is a carbamate hydrolase of Arthrobacter oxidans, which is responsible for the cleavage of the carbamate bond between the benzene rings of phenmedipham. This process includes the isolation of the carbamate hydrolase, the identification of the amino acid sequence of two BrCN cleavage peptides of the carbamate hydrolase, the synthesis of oligonucleotides for specific determination of the carbamate hydrolase sequence by hybridization and identification of the coding region, cloning and specifying the nucleotide sequence of the carbamate hydrolase gene from Arthrobacter oxidans.
Plants are transformed with recombinant genes coding for the carbamate hydrolase and transgenic plants which are tolerant to the herbicide are produced.

REFERENCES:
patent: 4810648 (1989-03-01), Stalker
Radin, et al (1978) Genet Res. (Camb.) 32:85-89.
Knowles, et al. (1981) Bull. Environ. Contam. Toxicol. 27:529-533.

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