Drug – bio-affecting and body treating compositions – Plant material or plant extract of undetermined constitution...
Reexamination Certificate
2000-10-02
2004-06-08
Lilling, Herbert J. (Department: 1651)
Drug, bio-affecting and body treating compositions
Plant material or plant extract of undetermined constitution...
C424S769000, C424S744000, C424S774000, C424S775000, C424S776000, C424S777000, C424S778000, C424S779000
Reexamination Certificate
active
06746694
ABSTRACT:
FIELD OF THE INVENTION
The present invention relates to the identification of a novel use of the principles extracted from the plant
Murraya koenigii
for treatment of asthma. This invention provides a process for the extraction of the principles from the plant
Murraya koenigii
. The invention also provides novel compositions useful in the treatment of asthma and methods for the treatment of asthmatic conditions using the said composition.
BACKGROUND AND PRIOR ART REFERENCES
Respiratory diseases such as asthma are reaching epidemic proportions in both the developed and developing world (Nature, Vol. 402, Supplement, and No. 6760, 1999. A special supplement on allergy and asthma). In countries such as Britain and Australia, the respiratory problem translates to 1 in 4 children under age of 14 years having asthma. The disease causes distress and misery in millions often at a time in their lives when they should be most active. Asthma interferes with sleep, intellectual functioning and recreational activities. At one extreme, asthma can be life threatening leading to occurrence to deaths that are avoidable.
Expert panel report has offered guidelines for diagnosis and management of asthma (JAMA Asthma Information Center, www.amaasssm.org/special/asthma/treatmnt/guide/guideline/gudelin.htm). Control of factors contributing to asthma severity is another avenue for management (JAMA Asthma Information Center, www.amaasssm.org/special/asthma/treatmnt/guide/guidelin/comp2/comp2.htm). several therapies and medications have also been suggested (JAMA Asthma Information Center, www.amaasssm.org/special/asthma/treatmnt/guide/guidelin/comp3/medicat/meditoc.htm and www.amaass.org/special/asthma/treatmnt/guide/guidelin/comp3/comp3.htm). Immunotherapy is another route for the treatment (JAMA Asthma Information Center, www.amasssm. or/special/asthma/treatmnt/guide/guidelin/comp2/immunoth.htm). Complementary alternative medicine is also being tried (JAMA Asthma Information Center, www.amaasssm.org/special/asthma/treatmnt/guide/guidelin/comp3/medicat/alternat.hmt) However, no satisfactory medication for cure of the disease has been found.
Herein the active factors in
Murraya koenigii
was prepared and used under in vitro system to show its usefulness for relief, treatment and cure of asthmatic problem. The plant
Murraya koenigii
has been studied and reported to have various medicinal values (Chakraborty, M. P Phytochemistry 1997, October; Khan B. A, Indian J of Expt. Biol. 1997 February; Khan B A Indian J of Physiology and Pharmacology 1996, April; Khan B A Plant Foods Hum. Nutr. 1996 June).
The leaf of this plant is widely used in various food preparations in India. Further, the plant is ubiquitous. Thus the prior art suggests that the extracts of the plant have several medicinal and other properties which have been widely investigated. This is adequate proof that the extracts of the plant can be safely consumed by humans without any side effects.
The inventors undertook a study on the extracts of this plant to identify the therapeutic principles of the plant. During their study, the inventories discovered the presence of the active principles in the leaf of
Murraya koenigii
which surprisingly were found to be useful in the treatment and cure of asthma.
OBJECTIVES OF THE INVENTION
The main objective of the present invention is to provide a method for the treatment of asthmatic conditions employing active principles extracted from the plant
Murraya koenigii.
Another object is to provide a process for the isolation of active principles from the plant
Murraya koenigii
and use the same for relief, treatment and cure of asthma.
Yet another object is to provide a simple, fast and inexpensive process to obtain a mixture of active (four) compounds possessing biological activities useful in the treatment of asthma.
Still another object is to provide novel compositions containing active principles extracted from the plant
Murraya koenigii
and useful in the treatment of asthma.
SUMMARY OF THE INVENTION
Accordingly, the present invention provides a method for the treatment of asthma, employing principles extracted from the plant
Murraya koenigii
. The invention also provides novel compositions useful in the treatment of asthma and methods for the extraction of active principles from the plant
Murraya koenigii.
DETAILED DESCRIPTION OF THE INVENTION
Accordingly, the invention provides a process for preparing an extract from the plant
Murraya koenigii
, useful in the treatment of asthma, said process comprising the steps of pulverising plant materials obtained from plant
Murraya koenigii
, extracting the plant material with a solvent at ambient temperature, concentrating the extract by filtering and evaporating it under reduced pressure and lyophilizing the concentrate to obtain a lyophilized extract containing active principles of the plant
Murraya koenigii.
In an embodiment, the plant materials are obtained from plant parts of
Murraya koenigii
selected from garden fresh leaves or leaves dried under shade.
In another embodiment, the leaves are pulverized by conventional methods to get homogenized leaves.
In yet another embodiment, the plant materials are extracted with solvents selected from hydrocarbon solvents, chlorinated solvents, ester solvents, ketonic solvents, alcohols, water and buffers.
In still another embodiment, the solvents are selected from the group consisting of petroleum ether (BP 40-60° C.), petroleum ether (60° C.-80° C.), benzene, pentane, hexane, chloroform, dichloromethane, carbon tetrachloride, diethyl ether, tetrahydrofuran, dioxane, acetone, cyclopentanone, ethyl acetate, ethyl formate, methanol, ethanol, n-butanol, water and buffers. In another embodiment, the concentration of the extract is effected by filtering and evaporating the solvents under reduced pressure at a temperature range of 20° C.-80° C. preferably at ambient temperature and lyophilizing the concentrate by conventional methods to obtain mixtures of the active factors.
In yet another embodiment, the extract obtained from the plant
Murraya koenigii
comprises active principles which dark colored solids soluble in dimethylsulfoxide.
In another embodiment, the active principles obtained from the plant
Murraya koenigii
are biocompatible and non-toxic in nature. The active principles have R
f
values 0.73, 0.60, 034 and 0.14 in chloroform and methanol in the ratio 19:1 and R
f
values 0.60, 0.38, 024 and 0.15 in the chloroform. The active principles have four peak with retention time 3.37, 3.49, 4.0 and 5.69 in methanol as solvent at 254 nm. The extraction process is carried out for a period ranging from 1-120 hrs, preferably between 12-16 hrs.
Further, the invention also provides pharmaceutical composition useful in the treatment of asthma, said composition comprising an effective amount of extract obtained from the plant
Murraya koenigii
together with, or optionally associated with a pharmaceutically acceptable additive. The additives used in the composition comprise powder or extracts of plants selected from
M. panicitlate Linn, H. abelmoschus, T ammi, S. aromaticum, A. vasica Nees, E hirta,
and
M. koinegii
. These additives are present in the range of 80-100 mg of
M. paniculate Linn,
40-60 mg of
H. abelmoschus,
38-62 mg of
T. ammi,
7-13 mg of
S. aromaticum,
85-115 mg of
A. vasica Nees
and 90-110 mg of
E hirta.
In an embodiment, the composition comprises
M. paniculata
Linn. Syn.
M exotica
90
mg
(KAMINI)
H. abelmoschus
50
mg
(JOWAN)
T. ammi
50
mg
(LAVANGA)
S. aromaticum
10
mg
(BASAK)
A. vasica
Nees
100
mg
(PUSITOA)
E. hirta
100
mg
M. koinegii
100
mg
(Suravi Neem)
In the said composition, the extract of the plant M. koiitegii is present in the range of 87-105 mg per dose. In the said composition, the extract of the plant
M. koinegii
comprises active principles which are dark colored solids, soluble in dimethylsulfoxide. The active principles have R
f
values 0.73, 0.60, 034 and 0.14 in chloroform and methanol in the ratio 19:1 and R
f
values 0.60, 0.38, 024 and 0. 15 in the c
Bhadra Ranjan
Bhattacharaya Samir
Chandra Pal Bikash
Das Krishna
Council of Scientific and Industrial Research
Lilling Herbert J.
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