Hepatoprotective agent of plant origin and a process thereof

Details Hepatoprotective agent of plant origin and a process thereof Hepatoprotective agent of plant origin and a process thereof

2006-01-24

2006-01-24

Tate, Christopher R. (Department: 1654)

Drug, bio-affecting and body treating compositions

Plant material or plant extract of undetermined constitution...

C514S025000, C514S183000, C514S185000, C514S732000, C514S893000

Reexamination Certificate

active

06989162

ABSTRACT:
A process of isolation of pure Acteoside of high hepatoprotection from plantColerbrookea oppositifolia, said process comprising steps of drying aerial parts of the plant, grounding the dried parts into powder, percolating the powder with water or ethanol for 3-4 times to obtain an extract, filtering the extract for clearing of suspended particles to obtain supernatant, drying the supernatant at about 45 to 55° C. to obtain a residue, fractionating the residue with chloroform, ethyl acetate, and butanol successively, subjecting butanol fraction to adsorption chromatography of SiO2after adding methanol to the fraction, charging the adsorbed fraction to glass column, eluting the column with solvents of increasing polarity of methanol:chloroform to obtain further fractions and repeating the process one more time, subjecting the fractions to column chromatography to obtain fractions, concentrating the fractions under reduced pressure to obtain acteoside as residue; and a method of effectively hepatoprotecting a subject using pure Acteoside from plantColerbrookea oppositifolia, said method comprising steps of administering appropriate low-dose of the acteoside to the subject.

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