Hepatitis E virus peptides and methods

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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435975, 436518, 530324, 530403, C12Q 170, G01N 33543, C07K 14005, C07K 1700

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active

056862393

ABSTRACT:
Peptide antigens are provided which are derived from the enterically transmitted non-A
on-B viral hepatitis agent, known as hepatitis E virus (HEV). The HEV derived peptides and in particular, SG3, are immunoreactive with sera from individuals infected with HEV. The antigens are useful as diagnostic reagents in diagnostic methods and kits for determining infection of an individual with HEV.

REFERENCES:
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Dawson, G.J., et al., "Solid-phase enzyme-linked immunosorbent assay for hepatitis E virus IgG and IgM antibodies utilizing recombinant antigens and synthetic peptides" J. Virol. Methods 38(1):175-86 (1992).
Huang, C.-C., et al., "Molecular Cloning and Sequencing of the Mexico Isolate of Hepatitis E Virus (HEV)", Virology 191(2):550-8 (1992).
Kaur, M., et al., "Human linear B-cell epitopes encoded by the hepatitis E virus include determinants in the RNA-dependant RNA polymerase" Proc. Natl. Acad. Sci. USA 89 (May):3855-8 (1992).
Khudyakov, Y.E., et al., "Epitope Mapping in Proteins of Hepatitis E Virus", Virology 194(4):89-96 (1993).
Lok, A.S.F., et al., "Comparison of Reactivity to ORF 2 and ORF 3 HEV Antigens in IgG and IgM Anti-HEV Assasy", International Symposium on Viral Hepatitis and Liver Disease --Scientific Program and Abstract Volume, Abstract 694, p. 262.
Purdy, M.A., et al., "Expression of a hepatitis E virus (HEV)-trpE fusion protein containing epitopes recognized by antibodies in sera from human cases and experimentally infected primates", Archives of Virology 123(3-4):335-49 (1992).
Reyes, G.R., et al., "Isolation of a cDNA from the Virus Responsible for Enterically Transmitted Non-A, Non-B Hepatitis", Science 247(4948):1335-9 (1990).
Tam, A.W., et al., "Hepatitis E Virus (HEV): Molecular Cloning and Sequencing of the Full-Length Viral Genome" Virology 185(1):120-31 (1991).
Tsarev, A., et al., "ELISA for Antibody Hepatitis E Virus (HEV) Based on Complete Open-Reading Frame-2 Protein Expressed in Insect Cells: Identification of HEV Infection in Primates" J. Infect. Dis. 168(2):369-78 (1993).
Yarbough, P.O., et al., "Hepatitis E Virus: Identification of Type-Common Epitopes", J. Virol. 65(11):5790-7 (1991).

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